HPLC-IT-TOF/MS分析地榆水煎液的化学成分群

Chemical constituent cluster of decoction of Sanguisorbae Radix by HPLC-IT-TOF/MS

目的基于HPLC-IT-TOF/MS系统地解析地榆水煎液的化学成分群。方法通过DAD检测器全波长扫描,联用ESI正负离子模式检测,根据精确分子质量及碎片信息、紫外光谱信息及极性对比,结合数据库信息。结果从地榆水煎液中共鉴定出82个成分(含同分异构体),包括69个酚类成分、8个三萜类成分及3个黄酮类成分、1个有机酸和1个单萜烯醇苷。其中柠檬酸、短叶苏木酚酸、甲氧基苯甲酸甲酯-5-O-硫酸酯、异鼠李素-硫酸酯、鞣花酸甲氧基衍生物-硫酸酯等物质在地榆中首次发现。结论系统地分析了地榆水煎液的成分群,进一步明确了地榆水煎液的物质基础,以期为地榆的药效和代谢研究提供科学依据。

Objective To analyze the chemical constituent cluster of decoction of Sanguisorba Radix systemically by HPLC-IT-TOF/MS. Methods The samples were scanned by broad spectrum of DAD detector and ionized in negative and positive environment of electron spray ionization. Results A total of 82 chemical constituents（include isomers） were identified based on exact molecular mass, fragment, ultraviolet spectrum as well as the combination of the database. The chemical constituent cluster was composed of 69 phenolics, 8 triterpenes, 3 flavonoids, an organic acid, and a monoterpene glycoside, of which citric acid, brevifolin carboxylic acid, methoxybenzoic acid methyl ester-5-O-sulfate, isorhamnetin-sulfate, and methylellagic acid-sulfate, et al were firstly found in Sanguisorba Radix. Conclusion The systemical analysis of the chemical constituent cluster of decoction of Sanguisorba Radix was able to supply a clear material basis for the further study of efficacy and pharmaceutical metabolism of Sanguisorba Radix.