一测多评法同时测定人参中人参皂苷Rb_1、Rg_1、Re、Rc、Rb_2、Rd

Simultaneous determination of six active components in Panax ginseng by QAMS method

目的建立可用于人参Panax ginseng中6个成分的一测多评法(QAMS)。方法采用高效液相色谱法,使用Phenomenex Luna C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸水为流动相,梯度洗脱,体积流量1.0 m L/min,柱温25℃,检测波长203 nm。以人参皂苷Rb_1为参照物,建立其与人参皂苷Rg_1、Re、Rc、Rb_2、Rd的相对校正因子,外标法与QAMS分别测定6个成分含量,t检验评价QAMS的可行性和适用性。结果在一定线性范围内,人参皂苷Rb_1相对人参皂苷Rg_1、Re、Rc、Rb_2、Rd的相对校正因子重复性好,2种方法所得6个成分的含量无明显差异,实验得相对校正因子可供参考。结论该法用于人参多种成分的同步质量评价是准确、可行的,可作为一种新的质量评价模式为中药及中药制剂实现多成分同时监控提供有益借鉴。

Objective This study aimed to simultaneously determine six composition of Panax ginseng by quantitative analysis of multi-components with a single-marker（QAMS） in different paris. Methods Phenomenex Luna C_（18）（250 mm × 4.6 mm, 5 μm） was used with mobile phase consisting of acetonitrile-0.1% phosphoric acid for gradient elution at a flow rate of 1.0 m L/min, The column temperature was 25 ℃ and the detection wavelength was 203 nm. Ginsenoside Rb_1 was used as reference to establish its relative correction factor of Rg_1, Re, Rc, Rb_2, Rd, The contents of six components were determined by both external standard method and QAMS. and t test was used to evaluate the feasibility and applicability of QAMS. Results In a certain linear range, the relative correction factor（RCF） was good, No significant differences were observed between the quantitative results of the two methods. Conclusion It is feasible and suitable to evaluate the quality of Panax ginseng. It can provide a useful reference to quality control of multi-indexed components in Chinese herbs and traditional Chinese preparations.