金黄色葡萄球菌生物膜形成能力及其耐甲氧西林相关性

Research on relationship between Methicillin resistance of Staphylococcus aureus clinical isolates and its biofilm forming ability

目的研究金黄色葡萄球菌临床分离菌株耐药性及其生物膜形成能力。方法采用药敏试验和人工生物膜培养方法,观察金黄色葡萄球菌耐药性及其生物膜形成能力。结果共检测201株临床分离的金黄色葡萄球菌,耐甲氧西林金黄色葡萄球菌(MRSA)为138株,占68.66%;甲氧西林敏感型金葡菌(MSSA)为52株,占25.87%。临床分离的金黄色葡萄球菌培养48 h后生物膜经结晶紫染色后的吸光度值MRSA与MSSA在生物膜形成能力方面无统计学差异。结论该医院临床分离的金黄色葡萄球菌中MRSA比例较高;可用结晶紫染色检测吸光度值的方法来测定生物膜形成能力。需进一步通过生物膜筛选法研究耐药性与生物膜形成能力之间的关系。

Objective To study the correlation between antibiotic susceptibility and biofilm-forming capacity of Staphylococcus aureus clinical isolates. Methods Drug sensitive test and artificial biological membrane cultivation method were used to observe the drug resistance and the biofilm formation ability of Staphylococcus. Results Of all 201 isolates 138 isolates were Methicillin-resistant Staphylococcus aureus which accounted for 68. 66%,while 52 isolates were MSSA that accounted for 25. 87%. No statistical difference was found between antibiotic susceptibility and biofilm-forming ability in these isolates by statistical analysis. Conclusion The drug resistance of Staphylococcus aureus isolates is serious. The quantitative method of testing adherent ability of Staphylococcus aureus can use the optical density measurement using crystal violet staining. The relationship between antibiotic susceptibility and biofilm adherent capability needs further study to be certificated and the bacterial biofilm screening tool is the next research goal.