摘要
目的探讨分析血管内皮生长因子受体(kinase insert domain containing receptor,KDR)阳性细胞在在心肌球样结构、血管和心肌成形中的作用。方法选择24只约250 g Wistar大鼠。消化分离心肌球源性细胞,流式细胞仪分析其免疫表型,免疫组化方法检测心肌与血管细胞的分化。结果 KDR阳性细胞体外呈指数生长并形成细胞团,并生成心肌前体细胞[肌钙蛋白T(cardiac troponin T,c Tn T)阳性],并可在体外培养条件下产生自主搏动。同时KDR阳性细胞还以出芽的方式形成许多血管状结构。结论 KDR阳性细胞在体外培养时能形成心肌球样结构,具有心肌和血管细胞分化潜能,是具有临床应用前景的细胞供体。
Objective To investigate the role of kinase insert domain containing receptor (KDR) positive cells in the formation of cardiospheric structure, myocardium and vessels. Methods Twenty-four Wistar rats weighting 250 g were selected. Cardiosphere-derived ceils were isolated by enzymatic digestion of rat hearts, and their immunological phenotypes were analyzed by using fluorescence-activated cell sorting (FACS). The cardiomyogenic and vasculogenic potential was diagnosed by immunohistochemistry. Results KDR positive ceils grew exponentially and formed cell clusters. It also could generate myocardial precursor cells (cardiac troponin T positive). And these cells can develop spontaneous contraction activity in vitro. Meanwhile, KDR positive cells formed many vessel-like structures through a budding process. Conclusion KDR positive cells form cardiospheric structure in vitro culture, and exhibit differentiation potential towards the cardiac and vascular cells. Therefore, KDR positive cells may have a broad prospect of clinicalapplication as cell donors.
作者
李莉
张蔚然
蒋小刚
韩志锋
刘超
刘磊
王晓明
LI Li, ZHANG Weiran, JIANG Xiaogang, HAN Zhifeng, LIU Chao, LIU Lei, WANG Xiaoming(Department of Cardiothoracic Surgery, BenQ Medical Center, the Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, 210019, P.R.Chin)
出处
《中国胸心血管外科临床杂志》
CAS
CSCD
2018年第4期333-337,共5页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
南京医科大学科技发展基金(2014NJMUZD026)
关键词
心肌球样结构
血管内皮生长因子受体
心肌成形
血管成形
心肌球源性细胞
Cardiospheric structure
kinase insert domain containing receptor
cardiogenesis
vasculogenesis
cardiosphere-derived cells