水解珍珠蜂蜜液活性成分的网络药理学分析

Network Pharmacology Analysis on Active Ingredients of Hydrolyzed Pearl Honey Liquid

目的：采用网络药理学思路和方法研究水解珍珠蜂蜜溶液，通过梳理“药材一化合物靶点．基因．疾病”的互相作用关系，探索其成分一靶点、成分-疾病和疾病一基因的关联性。方法：针对水解液中的有效成分珍珠和蜂蜜的化学活性成分，依据PubMed，TCMSP，STITCH数据库、国家人口健康科学数据共享平台，Gopubmed知识挖掘工具所获得的文献数据知识，试构建出多成分一多靶点网络、多成分一多疾病和疾病一基因网络，运用OMIM，CTD数据库中的呼吸系统相关疾病的基因和靶点数据进行富集分析，采用Cytoscape软件中Bisogenet插件建立疾病基因．蛋白质PPI关联网络模型。结果：对各种网络药理图的分析可以看出，这些化学活性成分可以作用560个靶点，224种不同类型的靶点中包含参与机体的肿瘤新生、血压调节和炎症反应等多种生理和病理过程的PTGSl和PTGS2两种异构酶，组织蛋白酶D（cathepsinD），凝血因子Ⅶ链（coagulation factor Ⅶ chain），重组人蛋白酪氨酸磷酸酶非受体1（PTPNl），细胞色素P450酶（cytochromeP450．cam，CYPs），氨基丁酸受体亚基-l（gamma-aminobutyric acid receptor subunit alpha-1，GABRAl）。结论：水解珍珠蜂蜜溶液有一定的抗炎作用，调节内分泌，收敛生肌作用，可用于治疗反复呼吸道感染，具有良好的应用前景；涉及到192种疾病，根据作者的临床基础，选取呼吸系统疾病的相关基因139个做通路富集分析，得到包含97个通路的通路集，为后续进行药效实验研究提供基础。

Objective： To study the hydrolytic pearl honey solution by using network pharmacology ideas and methods, and explore the correlations between compositions-targets, compositions-disease, and compositions- genes by analyzing the relationship of ＂ herb-compound targets-gene-disease＂. Method： Based on the literature data from PubMed, TCMSP, STITCH, database, the national population health science data sharing platform, and Go-pubmed knowledge mining tool, the effective chemical compositions of pearls and honey from the solution were used to construct the multi-composition-multi-target network, muhi-composition-muhi-disease network, and disease- gene network. Then the enrichment analysis of the genes and target data of respiratory system diseases in OMIM and CTD databases was conducted to build disease gene-protein PPI correlation network model by using Bisogenet plug- in components in cytoscape software. Result： The analysis of various network pharmacological maps showed that these chemical active components can play effect on 560 targets, 224 different types of targets included PTGS1 and PTGS2 isomerases involved in various physiological and pathological processes such as neoplasm, regulation of blood pressure, and inflammatory reaction in the body, cathepsin D, coagulation factor VH chain, recombinant human protein tyrosine phosphatase non-receptor 1 （PTPN1）, cytochrome P450-CAM （CYPs）, and Gamma aminobutyric acid receptor subunit alpha 1 （GABRA1）. Conclusion： It is suggested that the hydrolysis of pearl honey solution has some anti-inflammatory effects, regulating endocrine and astringent, which can be used to treat recurrent respiratory infection and has good application prospect. According to the author＇s clinical basis, 192 diseases were involved, and 139 genes related to respiratory disease were selected for pathway enrichment analysis, where 97 pathway sets were obtained to provide foundation for further experimental studies on efficacy.