低聚异麦芽糖调节肠道菌群及润肠通便作用的研究

Studies on the effects of isomaltooligosaccharides regulating intestinal microbial flora proliferation and relieving constipated function

为研究体外检测低聚异麦芽糖对长双歧杆菌和嗜酸乳杆菌生长的影响及低聚异麦芽糖对BALB/c小鼠肠道菌群影响以及对便秘小鼠润肠通便的作用。采用不同浓度低聚异麦芽糖与葡萄糖作为培养基中的碳源,与碳源为葡萄糖比较,检测不同浓度低聚异麦芽糖条件下长双歧杆菌和嗜酸乳杆菌的生长情况。体内实验以BALB/c雌性小鼠为实验对象,连续给予小鼠高剂量(1.0 g/(kg·bw))、低剂量(0.5 g/(kg·bw))的低聚异麦芽糖14 d后,测定小鼠粪便中双歧杆菌、乳杆菌、肠杆菌、肠球菌的含量;连续给予小鼠高、低剂量的低聚异麦芽糖21 d后,以复方地芬诺酯建立小鼠便秘模型,测定给予低聚异麦芽糖后便秘小鼠的排首黑便时间、6 h内黑便重量及小肠推进率。得到在体外实验中,与碳源为葡萄糖相比,碳源为不同浓度低聚异麦芽糖与葡萄糖时,长双歧杆菌与嗜酸乳杆菌的数量显著增加。体内实验中,实验组小鼠灌胃低聚异麦芽糖14 d后,与正常组相比,粪便中乳杆菌与双歧杆菌都极显著增加,B/E值也明显提高,且低剂量组中肠杆菌显著减少;灌胃前后自身相比,乳杆菌和双歧杆菌数量也显著增加。灌胃便秘小鼠低聚异麦芽糖后,其首粒排黑便时间明显缩短,排黑便总量明显增加,小肠推进率明显提高。体外培养条件下,低聚异麦芽糖可以增加长双歧杆菌和嗜酸乳杆菌的数量;低聚异麦芽糖具有改善小鼠胃肠道菌群及润肠通便的功效。

Objective ： The influence of isomaltooligosaccharide were tested for long bifidobacterium and lactobacillus acidophilus growth in vitro, and research as well as the influence of BALB/c mice intestinal runchang purge role for Constipated mice.Methods：by using different concentrations of isomaltooligosaccharide with glucose as carbon source in the culture medium, compared with only glucose as carbon source, detection under the condition of different concentrations of isomahooligosaccharide long growth situation of bifldobacterium and laetobacillus acidophilus.ln vivo experiments to BALB/c female mice as experimental object, giving mice with high dosage continuous （1.0 g/（ kg. bw））, low dose （0.5 g/（ kg·bw）） before and after 14 days of isomahooligosaccharide, determination of bifidobacteria in mouse droppings, lactobacillus, enterobacter and enterococcus content.Continuous give high and low doses of isomaltooligosaccharide in mice after 21 days,to compound diphenoxylate Constipated mice model was established, and determined to give isomaltooligosaccharide Constipated mice after the row of the first black black time, 6 hours and weight and small intestinal propulsion rates.Results：the in vitro experiment, compared with the carbon source is glucose, concentration of carbon source for different isomaltooligosaccharide and glucose, long the number of bifidobacterium and lactobacillus acidophilus increased significantly.In vivo experiment, the experimental mice stomach isomahooligosaccharide after 14 days, compared with normal group, lactobacillus and bifidobacterium in excrement and urine were significantly increased, B/E value also increased significantly, and low dose group of intestinal bacteria in significantly reduced. Lavage itself, compared before and after the number of lactobacillus and bifidobacterium also increased significantly.After lavage isomaltooligosaccharide Constipated mice, the first row of black time shortened obviously, obviously increase the total row of black and small intestinal propulsion rates increased significantly.Conclusion：under the culture condition in vitro, isomaltooligosaccharide can increase the number of bifidobacterium and lactobacillus acidophilus.Isomaltooligosaccharide is improve mice gastrointestinal flora and runchang purge efficacy.