铕原子标记技术对微量移液器容量误差的评估

Evaluation of pipettes performance by europium-labeled technique verified deviation

目的建立铕原子标记技术对实验室移液器容量的比对与校准,实现对小容量移液器容量准确度与精密度的有效监控。方法采用时间分辨荧光检测技术,以铕原子作为示踪材料,β-萘甲酰三氟丙酮为荧光增强液,以336～337nm为激发波长,610～613nm为测定波长,以荧光强度为观察指标,判断移液器容量的误差来源。结果时间分辨荧光法检测批内不精密在0.17%～7.8%之间;在即时、15min、30min、60min测定数据显示稳定,铕标记法与蒸馏水称重法对移液器容量测定的比对试验,相对误差在-3.9%～0.62%之间。结论时间分辨荧光进行移液器容量比对与误差评估,方法灵敏、精确、简便,可适用于临床实验室移液器容量的误差识别及常规比对。

Objective To establish a method for laboratorial pipette’s volume checking and calibration,which monitoring the precision and accuracy for the dispense of the micro pipettes volume effectively.Methods Using time-resolved fluoroimmunoassay (TR-FIA),europium atoms as a labeling material,β-naphthoyltrifluoroacetone as intensifier for fluorescence,wavelength between 336 to 337nm to explode and between 610 to 613nm to measure,fluorescent intensity as observational mark to verify the source of pipette’s volume deviation.Results In the group of TR-FIA,the inter-assay imprecision were 0.17%～7.8%;the data were steady when measured at once and after 15min,30min,60min.Comparing the results of europium-labeled method with distilled water gravimetric method,which shows that the relative deviation were -3.9%～0.62%.Conclusion TR-FIA for pipettes volume comparing and evaluation of deviation is sensitive,precise and convenient.This technique can be used for verified deviation and routine comparing of the pipette’s volume in clinical laboratory.