HCMV感染对多种机会性病毒感染的影响

The influence of HCMV infection on opportunistic virus infections

目的探究肾移植前人巨细胞病毒(human cytomegalovirus,HCMV)感染对移植后多种机会性病毒(HCMV、多瘤BK病毒(BK virus,BKV)、多瘤JC病毒(JC virus,JCV)、乙型肝炎病毒(hepatitis B virus,HBV)、丙型肝炎病毒(Hepatitis C virus,HCV))机会性感染的影响。方法采用免疫组化方法检测肾移植病人移植前后外周血HCMV被膜抗原pp65,巢式PCR方法检测病人尿液标本中的BKV DNA、JCV DNA;ELISA方法检测病人血液标本中的HBV抗原、抗体和HCV丙肝抗体(Anti-HCV);根据移植前有无感染HCMV将病人分为HCMV感染组和非感染组。结果 HCMV感染组与非感染组移植后HCMV、JCV、BKV、HBV、HCV感染率分别为20%与2.8%(P=0.049)、50%与19.4%(P=0.017)、0%与8.3%(P=0.545)、0%与13.9%(P=0.144)、0%与3.0%(P=1.000)。结论肾移植前HCMV感染不仅可引起移植后HCMV再激活感染,还可转化激活JCV的复制和感染。

Objective To investigate the impact of HCMV infection before transplantation on opportunistic virus infections after renal transplantation.Methods HCMV pp65 antigenemia was detected using immunohistochemical method of plasma;BKV DNA and JCV DNA were measured using nested qualitative polymerase chain reaction assays of urine;HBV antigen,antibody and anti-HCV were measured by enzyme linked immunosorbent assay(ELISA).According to whether HCMV infected or not before transplantation,all patients were divided into two groups(HCMV-infected group and un-infected group).Results The rates of HCMV,JCV,BKV,HBV and HCV infections were 20% vs 2.8%(P=0.049),50% vs 19.4%(P=0.017),0% vs 8.3%(P=0.545),0% vs 13.9%(P=0.144)and 0% vs 3.0%(P=1.000).Conclusion HCMV infection before transplantation not only has effect on HCMV reinfection,but also on JCV infection.