藏药大花龙胆正丁醇部位的化学成分研究

Chemical constituents of n-butanol fraction from Tibetan medicine Gentianae Szechenyii Spray

目的研究藏药大花龙胆Gentiana szechenyii花枝乙醇提取物的正丁醇部位的化学成分。方法通过制备高效液相色谱法分离纯化,利用各种谱学技术鉴定化合物的结构。结果从大花龙胆花枝乙醇提取物的正丁醇部位分离得到6个化合物,分别鉴定为大花龙胆酯苷A(1)、大花龙胆苷A(2)、异金雀花素-2″-O-吡喃葡萄糖苷(3)、异金雀花素(4)、乌奴龙胆苷D(5)和平龙胆酯苷(6)。结论化合物1为1个新的环烯醚萜苷类化合物,命名为大花龙胆酯苷A。

Objective To investigate the chemical constituents of n-butanol fraction of the ethanol extracts from spray of Gentianae szechenyii. Methods The chemical constituents were isolated and purified by repeated preparative high-performance liquid chromatography. The structures of the isolated compounds were identified on the basis of extensive spectroscopic techniques. Results Six compounds were obtained in n-butanol fraction of the ethanol extracts from G. szechenyii and identified as gentizechenlioside A（1）, szechenyin A（2）, isoscoparin-2″-O-glucopyranoside（3）, isoscoparin（4）, gentiournosides D（5）, and depressine（6）. Conclusion Compound 1 is a new iridoid glycoside which named as gentizechenlioside A.