羊踯躅的化学成分研究

Chemical constituents from fruits of Rhododendron molle

目的研究羊踯躅Rhododendron molle果实中的化学成分。方法利用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱、半制备高效液相、重结晶等多种方法进行分离和纯化,根据理化性质和波谱技术进行结构鉴定。结果从羊踯躅果实中分离得到15个化合物,鉴定其结构分别为杨梅素(1)、双氢杨梅素(2)、没食子儿茶素(3)、槲皮素-3′-O-葡萄糖苷(4)、儿茶素(5)、表儿茶素(6)、二氢槲皮素3′-O-葡萄糖苷(7)、原花青素A-2(8)、去氢双儿茶素A(9)、槲皮素-3-O-α-阿拉伯糖苷(10)、根皮苷(11)、南烛木树脂酚3-O-吡喃鼠李糖苷(12)、2,6-二甲氧-4-羟基苯酚-1-O-葡萄糖苷(13)、2,4,6-三羟基苯乙酮-2-O-吡喃葡萄糖苷(14)、5′-β-吡喃葡萄糖-O-茉莉酸(15)。结论化合物1~4、7~9、13~15首次从羊踯躅植物中分离得到,其中化合物1~11为黄酮类化合物。

Objective To investigate the chemical constituents from the fruits of Rhododendron molle. Methods Compounds were isolated and purified by a series of methods including silica gel, ODS column, Sephadex LH-20, semi-preparative HPLC, recrystallization, and so on. Their structures were identified by analysis of physico-chemical propertiesand spectroscopic technique. Results Fifteen known compounds were isolated and their structures were elucidated as myricetin（1）, dihydromyricetin（2）, gallocatechin（3）, quercetin-3′-O-glycoside（4）, catechin（5）, epicatechin（6）, taxifolin 3′-O-glucopyranoside（7）, proanthocyanidin A-2（8）, dehydroicatechin A（9）, quercetin-3-O-α-arabinoside（10）, phlorizin（11）, lyoniresinol 3-O-rhamnopyranoside（12）, 2,6-dimethoxy-4-hydroxyphenol-1-O-glucopyranoside（13）, 2,4,6-trihydroxy acetophenone-2-O-glucopyranoside（14）, and 5′-β-glucopyranosyloxy-jasmonic acid（15）. Conclusion Compounds 1—4, 7—9, and 13—15 are obtained from R. molle for the first time. Compounds 5, 6, and 10—12 are obtained from the fruits of this plant for the first time, of which compounds 1—11 are flavonoids.