青蒿琥酯对糖尿病大鼠视网膜MMP-9表达的影响

Effect of artesunate on expression of MMP-9 in rat retina

目的检测大鼠视网膜基质金属蛋白酶-9(MMP-9)及其抑制因子基质金属蛋白酶抑制剂-1(TIMP-1)表达量的变化,探讨青蒿琥酯在糖尿病视网膜病变过程中的作用。方法将24只大鼠随机分为对照组(n=6)、模型组(n=9)和青蒿琥酯组(n=9),采用链脲佐菌素(STZ)制备糖尿病大鼠模型,3个月后,给予青蒿琥酯ip 10 d后,摘除眼球,采用实时荧光定量PCR(RT-qPCR)法检测视网膜MMP-9及TIMP-1 mRNA相对表达量,免疫组化法检测MMP-9蛋白在视网膜组织中的表达情况。结果青蒿琥酯组大鼠视网膜MMP-9 mRNA的相对表达量明显低于模型组,而TIMP-1的相对表达量高于模型组,差异显著(P<0.05),免疫组化检测发现,青蒿琥酯组大鼠视网膜MMP-9的阳性表达率低于模型组(P<0.05)。结论青蒿琥酯具有抑制糖尿病视网膜病变MMP-9表达的作用。

Objective To investigate the effects of artesunate（ART） on diabetic retinopathy of rats by detecting the expression variation of matrixmetalloproteinase-9（MMP-9） and inhibitory factor matrix metalloproteinase inhibitor-1（TIMP-1） in retina. Methods A total of 24 healthy male SD rats were randomly divided into negative control, model control and ART treated groups. The model control group and ART treated group were given injection of streptozotocin（STZ） to establish the type 1 diabetic rat model. After being raised for three months, the ART treated rats’ abdomina were given ART injection for 10 d. All retinas of three groups were isolated. The expression of MMP-9 and TIMP-1 mRNA was detected by Real-time PCR（RT-qPCR） and the expression of MMP-9 protein was detected by immunohistochemical staining. Results The expression of MMP-9 in ART treated group was significantly decreased compared with model control group（P 〈 0.05）, whereas TIMP-1 in ART treated group was significantly increased（P 〈 0.05）. The immunohistochemical staining showed that the positive expression of MMP-9 protein in ART treated group was significantly less than that in model control group. Conclusion ART has inhibitory effects on the expression of MMP-9 in the diabetic retinopathy of rats.