木浆源甾醇对高脂高胆固醇仓鼠胆固醇代谢基因表达的影响

EFFECT OF WOOD PULP-DERIVED STEROLS ON CHOLESTEROL METABOLISM RELATED GENE EXPRESSION IN HAMSTERS FED A HIGH FAT AND HIGH CHOLESTEROL DIET

目的研究木浆源甾醇对高脂高胆固醇仓鼠肝脏中胆固醇代谢基因表达的影响。方法测定空白组、高脂组、实验组仓鼠的质量、脏器重量、血清中总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、甘油三酯(triglyceride,TG)水平。检测肝脏中胆固醇代谢相关调控基因m RNA表达水平及肝脏胆固醇含量和粪便固醇排泄量。结果木浆源甾醇可以极显著降低TC、TG、和Non HDL-C水平(P<0.01),同时极显著降低肝脏中胆固醇含量和肝脏重量(P<0.01),显著增加粪中固醇排泄量(P<0.05)。实验组肝脏中SREBP2、CYP7A1、HMG-Co A-R和LDL-R m RNA表达水平显著上调(P<0.05)。给予木浆源甾醇后可以改变肠道菌群多样性,增加有益菌减少有害菌数量。结论木浆源甾醇可能通过调控肝脏中胆固醇代谢相关基因的表达,抑制胆固醇的合成吸收并增加固醇的排泄,同时丰富肠道微生物多样性,增加有益菌数量,协同作用调控机体胆固醇的代谢。

Objective To study the effect of wood pulp-derived sterols on cholesterol metabolic gene expression in Golden Syrian hamsters fed a high-fat and high-cholesterol diet. Methods Serum total cholesterol （TC）, triglyceride （TG） and high density lipoprotein cholesterol （HDL-C） were detected in normal group, high fat group and experimental group. The expression of cholesterol-regulating genes in the liver was assessed, and the contents of cholesterol in the liver, fecal neutral and acidic sterols were determined. Results Wood pulp-derived sterols could significantly decrease TC, TG and Non HDL-C levels （P〈0.01） and significantly decrease the weight of liver and content of cholesterol in the livers （P〈0.01）. It also could increase fecal steroid excretion （P〈0.05）. The mRNA expression levels of sterol regulatory element-binding protein 2 （SREBP2）, cytochrome P450 7A1（CYP7A1）, HMG-CoA reductase （HMG-CoA-R）, and low density lipoprotein receptor （LDL-R） in the liver of the experimental group were increased （P〈0.05）. The wood pulp-derived sterols supplementation could change the diversity of intestinal microbiota, increase the number of bacteria and reduce harmful bacteria. Conclusion Wood pulp-derived sterol could inhibit cholesterol absorption and synthesis, increase cholesterol excretion, enrich intestinal microbial diversity, increase the number of bacteria, and regulate cholesterol metabolism through regulating cholesterol metabolism gene expression in the liver.