脂肪酸合成通路涉及Wolbachia介导的伊蚊抗登革病毒作用

Fatty acid synthesis pathway is involved in Wolbachia-mediated DENV inhibition in Aedes

目的评价沃尔巴克氏体(Wolbachia)介导的埃及伊蚊抗登革病毒作用,探讨脂肪酸合成通路与Wolbachia介导的伊蚊抗登革病毒作用的关系。方法检测本实验室建立的稳定携带wAlbB型Wolbachia的埃及伊蚊株(GDB)Wolbachia的组织分布情况;检测该蚊株对登革2型病毒的易感性;比较GDB株和埃及伊蚊广东株(GD)成蚊脂肪酸合酶(FAS)基因的表达水平;给予GDB株补充外源性脂肪酸,再感染登革2型病毒,检测病毒复制水平是否发生改变。结果与埃及伊蚊GD株比较,GDB株埃及伊蚊对登革2型病毒感染具有显著抑制作用,差异有统计学意义(P=0.001);GDB株成蚊FAS基因的表达水平显著低于GD株,差异有统计学意义(P=0.002);GDB株得到外源性脂肪酸补充后,其病毒复制水平显著升高,差异有统计学意义(P=0.001)。结论携带wAlbB型Wolbachia的埃及伊蚊株对登革病毒感染的抑制作用与蚊虫的脂肪酸合成通路受到抑制有关。

Objective To evaluate the anti-dengue virus effect mediated by Wolbachia in Aedes aegypti and to explore the relationship between the fatty acid synthesis pathway and Wolbachia-mediated anti-dengue virus infection. Methods wAlbB- infected Aedes aegypti Guangdong Strain （GDB） was generated via embryo microinjection. The distribution of wAlbB in the mosquito tissues was detected. The expression level of the fatty acid synthase gene and DENV-2 replication level in GDB and GD were compared. The dengue virus replication level in the GDB strain fed with exogenous fatty acids was measured. Results The Wolbachia-infected Ae. aegypti was resistant to DENV-2 infection （P=0.001） with a lower level of the FAS gene expression （P=0.002）, and the virus replication level was increased when the GDB strain was fed with exogenous fatty acids （P=0.001）. Conclusion The inhibitory effect of the wAlbB Wolbachia in GDB on DENV-2 infection was related to the mosquito fatty acid synthesis pathway.