不同亚群滤泡辅助性T细胞在骨肉瘤患者中的数量变化及临床意义

Frequencies and correlation of different follicular helper T cell subsets in osteosarcoma

滤泡辅助性T细胞(follicular helper T,Tfh)在多种肿瘤疾病的发病机制中发挥着重要作用,然而骨肉瘤疾病的发病过程是否有Tfh的参与尚不清楚。为了研究外周血Tfh在骨肉瘤发病过程中的变化及其与疾病进展的关系,本研究运用流式细胞术,测定骨肉瘤患者和健康对照组外周静脉血中不同Tfh亚群的数量。课题组发现骨肉瘤患者外周血中存在较多数量的PD-1+Tfh、ICOS+Tfh及IL-21+Tfh,有趣的是课题组发现骨肉瘤患者外周血中PD-1+Tfh的数量随着肿瘤分期等级的增高呈递增的趋势。这提示Tfh在骨肉瘤的发病过程中发挥着重要作用,PD-1+Tfh与疾病进展有密切的关系,可以作为骨肉瘤疾病进展的一种生物学指标。

To study the role in the pathogenesis of follicular helper T（Tfh）cells in osteosarcoma（OS）,we measured the frequencies of peripheral blood inducible Tfh cells in OS patients and healthy controls by flow cytometry.At the same time,the disease progression of OS patients was quantified.We found higher frequencies of PD-1^＋Tfh,ICOS^＋Tfh,and IL-21^＋Tfh cells in OS than those in controls;Interestingly,we also found the frequencies of PD-1^＋Tfh cells were positively associated with the staging of osteosarcoma.In conclusion,Tfh cells may participate in the pathogenesis of osteosarcoma,and increased PD-1^＋Tfh cells may be associated with the progression of osteosarcoma.