高效液相色谱法同时测定化妆品中的12种防腐剂

Simultaneous determination of 12 kinds of preservatives in cosmetics by high performance liquid chromatography

采用高效液相色谱法同时测定不同种类化妆品中12种防腐剂的含量。样品用甲醇提取,经Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm)分离,流动相采用乙腈-0.1%磷酸溶液梯度洗脱,用280和254 nm波长同时进行检测,柱温30℃。结果表明,12种防腐剂在进样范围内线性关系良好,相关系数均大于0.996;4种不同基质中低(60%)、中(100%)、高(140%)3个梯度的加样回收率为95.2%~112.1%,RSD为0.36%~2.64%(n=9)。40批次洗护类样品的测定结果表明方法具有较高的重现性和耐用性,可用于同时测定化妆品中12种防腐剂的含量。

HPLC method was established for simultaneous determination of 12 kinds of preservatives in cosmetics. Samples were extracted by methanol,then separated by Phenomenex Luna C18 column（ 4. 6 mm ×250 mm,5 μm） using acetonitrile and 0. 1% phosphoric acid as mobile phase with gradient elution,the detection wavelengths were 280 and 254 nm,and the column temperature was 30 ℃. The calibration curves shows good linear relationship in the range of injection volume and all the correlation coefficients are above 0. 996,the average recoveries spiked at 60%,100%,140% are in the range of 95. 2%-112. 1% with RSD of 0. 36%-2. 64%（ n = 9）. The method shows good reproducibility and durability through detection of 40 batches of samples. It can be used for simultaneous determination of 12 kinds of preservatives in cosmetics.