摘要
采用高效液相色谱法同时测定不同种类化妆品中12种防腐剂的含量。样品用甲醇提取,经Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm)分离,流动相采用乙腈-0.1%磷酸溶液梯度洗脱,用280和254 nm波长同时进行检测,柱温30℃。结果表明,12种防腐剂在进样范围内线性关系良好,相关系数均大于0.996;4种不同基质中低(60%)、中(100%)、高(140%)3个梯度的加样回收率为95.2%~112.1%,RSD为0.36%~2.64%(n=9)。40批次洗护类样品的测定结果表明方法具有较高的重现性和耐用性,可用于同时测定化妆品中12种防腐剂的含量。
HPLC method was established for simultaneous determination of 12 kinds of preservatives in cosmetics. Samples were extracted by methanol,then separated by Phenomenex Luna C18 column( 4. 6 mm ×250 mm,5 μm) using acetonitrile and 0. 1% phosphoric acid as mobile phase with gradient elution,the detection wavelengths were 280 and 254 nm,and the column temperature was 30 ℃. The calibration curves shows good linear relationship in the range of injection volume and all the correlation coefficients are above 0. 996,the average recoveries spiked at 60%,100%,140% are in the range of 95. 2%-112. 1% with RSD of 0. 36%-2. 64%( n = 9). The method shows good reproducibility and durability through detection of 40 batches of samples. It can be used for simultaneous determination of 12 kinds of preservatives in cosmetics.
作者
韩晓萍
李亚楠
周霈
HAN Xiao - ping1,2, LI Ya - nan1 , ZHOU Pei1(1. Qinghai Institute for Drug Control,Xining,Qinghai 810016,China; 2. Qinghai Key Laboratory of Modernization Research of TCM and Tibetan Medicine,Xining,Qinghai 810016 ,Chin)
出处
《日用化学工业》
CAS
CSCD
北大核心
2018年第3期178-182,共5页
China Surfactant Detergent & Cosmetics
基金
青海省药品检验检测平台(2017-ZJ-Y40)
关键词
化妆品
高效液相色谱法
防腐剂
cosmetics
high performance liquid chromatography
preservative