82株副溶血性弧菌毒力基因及耐药性分析

Analysis of virulence genes and drug resistance in 82 Vibrio parahaemolyticus strains

目的了解2015—2016年南通市副溶血性弧菌分离株毒力基因携带与耐药性情况，为防治副溶血性弧菌病及指导临床合理用药提供基础数据。 方法利用荧光定量PCR技术，对副溶血性弧菌进行不耐热溶血素基因（tlh）、耐热直接溶血素基因（tdh）和耐热相关溶血素基因（trh）毒力基因检测。采用微量肉汤稀释法，测定其对15种抗生素的耐药性。结果82株副溶血性弧菌，tlh均为阳性，阳性率为100.0%（82/82）；tdh阳性72株，阳性率为87.8%（72/82）；全部菌株trh基因阴性。82株副溶血性弧菌，对氨苄西林、头孢唑啉、四环素、氯霉素的耐药率均为1.2%（1/82），对甲氧苄氨嘧啶/磺胺甲噁唑的耐药率为2.4%（2/82），对另外10种抗生素敏感或中介。结论2015—2016年南通市副溶血性弧菌分离株主要携带毒力基因tlh、tdh，不携带trh基因。除氨苄西林、头孢唑啉、四环素、氯霉素、甲氧苄氨嘧啶/磺胺甲噁唑这5类抗生素外，其余10类抗生素在副溶血性弧菌感染的治疗中都有效，可作为首选抗生素。

Objective To analyze the virulence genes and drug resistance in Vibrio parahaemolyti- cus strains isolated in Nantong City from 2015 to 2016 in order to provide reference for the prevention and treatment of Vibrio parahaemolyticusinfection and for rational use of medicines. Methods Virulence genes of tlh, tdh and trh in Vibrio parahaemolyticus strains were detected by fluorescence quantitative PCR. Micro- broth dilution method was used to analyze antimicrobial resistance in these strains to 15 kinds of antibiotics. Results Eighty-two Vibrio parahaenmlyticus strains were all positive for tlh gene and negative for trh gene and among them, 72 carried tdh gene （87.8%）. Antimicrobial resistance rates of these strains to ampicil- lin, cefazolin, tetracycline and chloramphenicol were all 1.2% （1/82）. Two strains （2.4%） were resist- ant to trimethoprim/sulfamethoxazole. All strains were sensitive or intermediate to another 10 kinds of antibi- otics. Conclusion From 2015 to 2016, Vibrio parahaemolyticus strains carrying virulence genes of tlh and tdh were prevalent in Nantong and no trh gene-positive strains were reported. Except ampicillin, cefazolin, tetracycline, ehlorarnphenieol and trimethoprim/sulfamethoxazole these five kinds of antibiotics, the remai- ning 10 kinds of antibiotics were effective against Vibrio parahaemolyticus and could be used as the treatment of choice.