铜绿假单胞菌感染后人脐带间充质干细胞对A549细胞凋亡的保护作用研究

Protective effect of human umbilical cord mesenchymal stem cells on apoptosis of A549 cells after Pseudomonas aeruginosainfection

目的观察感染状态下应用人脐带间充质干细胞(hUCMSCs)对A549细胞凋亡起到的保护作用。方法无菌条件下从胎儿脐带和壁蜕膜组织中采集hUCMSCs标本,接种铜绿假单胞菌(PA)于A549细胞以建立A549细胞损伤模型,分析PA对A549细胞凋亡的影响及hUCMSCs对细胞凋亡的保护。结果经PA感染后,导致A549细胞生长缓慢、老化,细胞空泡化;感染状态下A549细胞SP-A表达水平下降,与未感染A549细胞相比差异明显(t=7.745,P=0.001);感染A549细胞接种hUCMSCs后,SP-A表达水平上调,与感染组相比差异明显(t=3.872,P=0.017);PA感染后A549细胞凋亡率加快,存活率降低,接种hUCMSCs后A549细胞凋亡率下降,存活率上调。结论 A549细胞在PA感染状态下通过接种hUCMSCs,可起到修复细胞损伤,抑制细胞凋亡的作用。

OBJECTIVE To observe the protective effect of human umbilical cord mesenchymal stem cells（hUCMSCs）on apoptosis of A549 cells of patients with infections.METHODS The hUCMSCs specimens were collected from fetal umbilical cord and decidua tissues under aseptic condition,the A549 cells were inoculated with Pseudomonas aeruginosa so as to establish A549 cell damage model,and the impact of P.aeruginosa on the apoptosis of the A549 cells and the protective effect of hUCMSCs on the apoptosis were observed.RESULTS The P.aeruginosa infection resulted in the slow growth,aging and vacuolation of the A549 cells,the expression of SP-A in the A549 cells was reduced under the infection state,as compared with the A549 cells that were not infected,there was significant difference（t=7.745,P=0.001）.The expression of SP-A in the A549 cells was up-regulated after the inoculation of hUCMSCs,as compared with infection group,there was significant difference（t=3.872,P=0.017）.The rate of apoptosis of the A549 cells accelerated after the P.aeruginosainfection,and the survival rate was reduced;the rate of apoptosis of the A549 cells was reduced after the inoculation of hUCMSCs,and the survival rate was up-regulated.CONCLUSIONThe inoculation of hUCMSCs may repair the damage of the A549 cells infected with P.aeruginosainfection and inhibit the apoptosis.