芝麻素对实验性脑出血后脑损伤的保护作用

Protection effect of sesamin on brain damage after experimental intracerebral hemorrhage

目的探讨芝麻素对实验性脑出血后继发脑损伤的保护作用。方法采用自体血脑出血模型,随机将小鼠分为假手术组、脑出血组、脑出血后注射芝麻素组。称量脑组织干湿重来观察脑水肿的变化;通过伊文思蓝渗出量评估血-脑屏障的破坏;同时用免疫荧光双标和Western blot方法检测血-脑屏障结构中紧密连接蛋白的表达改变;采用实时荧光定量PCR检测脑出血周围组织中炎症因子IL-1、IL-6和TNF-α的表达水平。结果芝麻素显著减轻了脑出血后脑组织的水含量(P<0.05);减少了伊文思蓝的渗出量(P<0.001)和紧密连接蛋白的降解;并降低了炎症因子IL-1β、IL-6和TNF-α的表达水平(均P<0.001)。结论芝麻素可能是通过抑制炎症反应,改善血-脑屏障的破坏,减轻脑水肿,从而保护脑出血后继发脑损伤。

Objective To explore the protection of sesamin in brain damage after experimental intracerebral hemorrhage. Methods Manufacture of autologous blood cerebral hemorrhage model in mice. Rats were randomly divided into sham operation group,cerebral hemorrhage group and the injection of sesamin in cerebral hemorrhage group. The changes of brain edema by weighing tissue dry wet weight were observed. Blood-brain barrier damage by the evans blue effusion quantity were evaluated. The expression of tight junction protein in the blood brain barrier by double-labelling immunofluorescence and western blot were detected. The expression of inflammation factor IL-1β,IL-6,and TNF-α in cerebral hemorrhage by Real-time fluorescent quantitative PCR were detected.Results Sesamin significantly reduced the severity of brain edema of cerebral hemorrhage（ P 0. 05）. The effusion quantity of evans blue（ P 0. 001） and the degradation of tight junction protein after cerebral hemorrhage were reduced. The structural integrity of the blood-brain barrier were improved. The inflammatory response after cerebral hemorrhage were suppressed. Conclusion Sesamin probably protect the brain injury after intracerebral hemorrhage by inhibiting the inflammatory reaction,improve the blood brain barrier damage and reduce brain edema.