OIP5对肝癌细胞SMMC-7721增殖和侵袭迁移能力的影响

Effects of OIP5 on the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cell line SMMC-7721

目的:探讨OIP5对肝癌细胞SMMC-7721增殖和侵袭迁移能力的影响。方法:采用RNA干扰技术沉默肝癌细胞中OIP5的表达后,通过qRT-PCR和Western-blot技术检测OIP5的下调效率,CCK-8和平板克隆法检测肝癌细胞的增殖能力,Transwell法检测肝癌细胞的侵袭和迁移能力。结果:转染OIP5-siRNA后,肝癌细胞SMMC-7721中OIP5 mRNA和蛋白的表达水平均明显降低(P<0.05);同时,与对照组相比,OIP5-siRNA组肝癌细胞SMMC-7721的CCK-8实验的OD值、平板克隆法测得的克隆球个数、Transwell法测得的迁移细胞数与侵袭细胞数均明显低于对照组(P<0.05)。结论:OIP5能够促进肝癌细胞的增殖和侵袭迁移,可能作为肝癌治疗的潜在靶点。

Objective： To investigate the protective effects of OIP5 on the proliferation, migration and invasion of hepatocellular carcinoma （HCC） cell line SMMC-7721. Methods： OIP5 was down-regulated by using small interfering RNA in HCC cell line SMMC-7721. The transfection efficiency of OIP5-siRNA was detected with qRT-PCR and Western blot. The proliferation of HCC cells was detected by CCK-8 and colony formation assay, and the migration and invasion of HCC cells were measured by Transwell assay.Results： The level of OIP5 mRNA and protein were significantly decreased after being transferred by OIP5-siRNA in SMMC-7721 cells.After knockdown of OIP5, the OD value measured by CCK-8, the number of conoly, metastasis and invaded cell number were significantly decreased compared with the control group（P0.05）. Conclusions： OIP5 can promote the proliferation, invasion and metastasis of HCC SMMC-7721 cells, and targeting OIP5 is likely to be a promising strategy for the treatment of HCC.