高龄Ⅲ型前列腺炎患者血清和前列腺液中神经生长因子、炎性因子的表达水平及临床意义

Expressions and Clinical Significances of Nerve Growth Factor and Inflammatory Factors in Serum and Expressed Prostatic Secretions in the Elderly Patients with Prostatitis

目的:探讨高龄Ⅲ型慢性前列腺炎(CP)患者血清和前列腺液(EPS)中神经生长因子(NGF)、炎性因子的表达水平及其临床意义。方法:收集2015年5月~2016年12月我院收治的150例高龄Ⅲ型CP患者(CP组),其中ⅢA型81例,ⅢB型69例,另选择150例健康体检者作为对照(对照组)。采用双抗体夹心酶联免疫吸附法(ABC-ELISA)检测其血清、EPS中NGF、IL-8和TNF-α的表达水平,分析NIH-CPSI、NGF、IL-8和TNF-α之间的相关性。结果:CP组血清、EPS中TNF-α、IL-8、NGF水平均明显高于对照组(P<0.05);与IIIB型患者比较,IIIA型患者TNF-α、IL-8水平明显升高(P<0.05);EPS中TNF-α、IL-8、NGF水平均明显高于血清(P<0.05)。IIIB型患者的NIH-CPSI症状评分明显低于IIIA型(P<0.05)。CP患者EPS中NGF和TNF-α、IL-8均呈显著正相关(P<0.05);血清中NGF和TNF-α、IL-8亦呈显著正相关(P<0.05)。III型CP患者血清、EPS中,TNF-α、IL-8、NGF与NIH-CPSI呈显著正相关(P<0.05)。结论:高龄III型CP患者血清、EPS中TNF-α、IL-8、NGF表达水平明显升高,且与NIH-CPSI明显相关,联合检测有助于评估III型CP患者的病情严重程度。

Objective： To explore the expressions and clinical significances of nerve growth factor（NGF） and inflammatory factors in serum and expressed prostatic secretions（EPS） in the elderly patients with chronic prostatitis（CP）. Methods： Patients with Type Ⅲ CP（CP group） who were admitted from December 2013 to December 2016 and 150 cases of healthy controls were selected. CP group included 81 cases of Type Ⅲ A CP, 69 cases of Type Ⅲ B CP. The NGF, IL-8 and TNF-α levels in serum and EPS were detected by ABC-ELISA, and the correlation of NIH-CPSI, NGF, IL-8 with TNF-α were analyzed. Results： The levels of TNF-α, IL-8 and NGF in serum, EPS in CP group were significantly higher than those of the control group（P0.05）. Compared with the type IIIB CP, the levels of TNF-α, IL-8 in type IIIA CP were significantly increased（P0.05）. The levels of TNF-α, IL-8 and NGF in EPS were significantly higher than those in the serum（P0.05）. The NIH-CPSI symptom scores of patients with type IIIB CP was significantly lower than that of patients with type IIIA CP（P0.05）. The levels of TNF-α, IL-8 in serum and EPS of CP patients had significantly positive correlation with NGF（P0.05）. There was positive correlation of TNF-α, IL-8, NGF with NIH-CPSI in serum and EPS of CP patients（P0.05）.Conclusions： The levels of TNF-α, IL-8, NGF in serum and EPS of elderly patients with type III CP were highly expressed and closely correlated with the NIH-CPSI. Combination detection of TNF-α, IL-8, NGF could contribute to evaluate the severity of CP.