基于金属光栅近场衍射增强的结构光照明技术

Near-Field Diffraction Enhanced Structured Illumination Microscopy Based on Metallic Grating

生命科学领域的研究需要对样本进行纳米量级的宽视场、实时动态观测。提出了一种基于金属光栅近场衍射增强的结构光照明技术,该技术在传统e-pi显微镜的基础上引入金属光栅和空间光调制器;对该技术的亚波长成像性能进行了理论分析和数值分析。研究结果表明:在波长为520nm、数值孔径为1.3的条件下,该技术可以使半峰全宽的横向空间分辨率为65nm,相比于宽场照明显微分辨率提升了约3.7倍;该技术以其优异的亚波长成像性能在生命科学领域研究中具有潜在的应用价值。

Biological research requires dynamic and wide-field optical microscopy with nanoscale resolution to study the biological process. To address this issue, a structured illumination microscopy based on near-field diffraction enhancement using metallic grating is proposed. The technique introduces spatial light modulator and metal grating on the basis of the conventional e-pi microscope. The sub-wavelength imaging performance of this technique is studied theoretically and numerically. The results show that the transverse spatial resolution of full width at half maximum is 65 nm at wavelength of 520 nm and numerical aperture of 1.3. Compared with the traditional wide- field microscopy, the resolution of the technology increases by about 3.7 times. This technology has potential application in biological research.