红花提取液对小鼠缺血再灌注脑损伤保护作用的实验研究

Protective Effect of Honghua( Safflower) Extract on Cerebral Ischemia-Reperfusion Injury in Mice

目的:探讨红花提取液对小鼠急性脑缺血损伤的保护作用。方法:将ICR小鼠随机分为假手术组,模型组,红花提取液低、中、高剂量组(2.5、5.0、10.0 g/kg)以及阳性对照药组(尼莫地平10 mg/kg),造模前连续给药7 d;采用线栓法建立小鼠脑缺血再灌注模型,再灌注24 h后,测定神经功能评分、脑含水量、脑梗死体积及脑组织SOD、GSH-Px、CAT活力及MDA含量。结果:红花提取液能显著促进MCAO小鼠神经功能缺损症状的恢复;降低小鼠脑缺血再灌注后脑梗死体积和脑含水量;红花提取液中、高剂量组均能显著提高脑组织SOD、GSH-Px、CAT活力,降低MDA含量。结论:红花提取液对小鼠急性脑缺血损伤具有保护作用,且其保护作用可能与其抗氧化应激相关。

Objective： To explore the protective effect of Safflower Extract on acute cerebral ischemic injury in mice. Methods： ICR mice were randomly divided into sham operation group, model group, low, middle and high dose groups （2.5, 5. 0, 10 g/kg） and positive control group. The model of focal cerebral ischemia was es- tablished by modified Zea Longa method. After 6 hours, the neurological function scores, brain water content, cerebral infarction volume were determined and SOD, GSH -Px , CAT SOD activities and MDA content in brain tissue were determined. Results： Safflower Extract could promote neurological function of MCAO mice recover, reduce cerebral infarction volume and brain water content; middle and high dose Safflower Extract could raise SOD , GSH -Px and CAT activities in brain tissue and lower MDA content. Conclusion：Safflower Extract can protect mice＇ cerebral tissue from ischemic injury, that is related to anti -oxidative effect.