TIRC7在慢性髓系白血病患者单核细胞中的表达

Expression of TIRC7 in mononuclear cells in patients with chronic myeloid leukemia

目的探讨T细胞免疫应答cDNA7(TIRC7)在慢性髓系白血病(CML)患者单核细胞(MNC)中的表达水平。方法收集24例CML患者(CML组)及6例健康志愿者(对照组)的新鲜骨髓或外周血,采用琼脂糖凝胶电泳及实时荧光定量PCR法检测TIRC7基因在MNC中的表达水平。结果应用TIRC7引物可扩增出大小约210bp的片段,与理论值相符。CML组TIRC7扩增条带亮度较弱,而对照组TIRC7条带明亮。CML组TIRC7相对表达量为0.085(0.032,0.197),低于对照组的0.960(0.715,2.027)(P<0.01)。结论 TIRC7在CML患者MNC中的表达水平较低,可能在CML的发生、发展中起重要作用。

Objective To investigate the expression of T-cell immune response cDNA 7（TIRC7）in mononuclear cells（MNC）of patients with chronic myeloid leukemia（CML）.Methods The fresh bone marrow or peripheral blood was collected from 24 CML patients（group CML）and 6 healthy people（group C）.The expression of TIRC7 in MNC was detected by agarose gel electrophoresis and real-time quantitative PCR.Results The 210 bp fragment was amplified by TIRC7 primer and coincided with theoretical value.The stripe in group CML was weaker and that in group C was brighter.The relative expression of TIRC7 was lower in group CML than that in group C（P〈0.01）.Conclusion The expression of TIRC7 is lower in MNC of CML patients,which may be related to the development of CML.