程序性死亡配体-1在鼻咽癌组织中的表达及临床意义

Expression of Programmed Cell Death Ligand-1 in Nasopharyngeal Carcinoma and Its Clinical Significance

目的:探讨程序性死亡配体-1(PD-L1)在鼻咽癌组织中的表达及临床意义。方法:选取2015年7月-2017年6月期间在我院接受治疗的鼻咽癌患者333例,收集其鼻咽癌组织作为观察组标本,另选取同期在我院接受治疗的慢性鼻咽炎患者102例的鼻咽炎组织作为对照组标本。采用免疫组化法和逆转录-聚合酶链反应(RT-PCR)检测两组患者鼻咽组织中PD-L1蛋白和PD-L1 mRNA的表达,并分析观察组患者鼻咽癌组织中PD-L1蛋白和PD-L1 mRNA的表达与临床病理参数的关系。结果:对照组患者鼻咽炎组织中PD-L1蛋白的阳性率为0.00%(0/102),观察组患者鼻咽癌组织中PD-L1蛋白的阳性率为69.37%(231/333),两组PD-L1蛋白的阳性率比较差异有统计学意义(P<0.05)。RT-PCR结果显示,对照组患者鼻咽炎组织中未见PD-L1 mRNA表达,观察组患者鼻咽癌组织中PD-L1 mRNA的相对表达水平为(0.82±0.27),差异有统计学意义(P<0.05)。观察组患者鼻咽癌组织中PD-L1蛋白和PD-L1 mRNA的表达与年龄、性别无关(P>0.05),而TNM分期为Ⅲ-Ⅳ期、有淋巴结转移、有吸烟史患者鼻咽癌组织中PD-L1蛋白阳性率和PD-L1 mRNA的表达均高于TNM分期Ⅰ-Ⅱ期、无淋巴结转移、无吸烟史患者(P<0.05)。结论:在鼻咽癌组织中PD-L1蛋白和PD-L1 mRNA呈现高表达,且其表达与TNM分期、淋巴结转移、吸烟史有关。

Objective： To investigate the expression and clinical significance of programmed cell death ligand-1（PD-L1） in nasopharyngeal carcinoma（NPC）. Methods： A total of 333 patients with nasopharyngeal carcinoma,who were treated in the First People＇s Hospital of Yulin from July 2015 to June 2017, were collected; the nasopharyngeal carcinoma tissues were collected as observation group, 102 cases of chronic pharyngitis during the same period were taken as control group.Immunohistochemical staining and reverse transcription polymerase chain reaction（RT-PCR） were used to detect the expression of PD-L1 and PD-L1 mRNA in the nasopharynx tissues of two groups. The relationship between the expression of PD-L1 protein and PD-L1 mRNA and the clinicopathological parameters in the patients with nasopharyngeal carcinoma were analyzed. Results： The positive rate of PD-L1 protein in the nasal pharyngitis tissue of the control group was 0.00%（0/102）, the positive rate of PD-L1 protein in the nasopharyngeal carcinoma tissues of the observation group was 69.37%（231/333）; the positive rates of PD-L1 protein in the two groups were statistically significant（P〈0.05）. The results of RT-PCR showed that there were no expressions of PD-L1 and mRNA in the control group, the relative expression of PD-L1 and mRNA in the nasopharyngeal carcinoma tissues of the observation group was（0.82＋0.27）, the difference was statistically significant（P〈0.05）.The expressions of PD-L1 protein and PD-L1 mRNA in the nasopharyngeal carcinoma tissues of the observation group were not related to age and sex（P〈0.05）. The positive rate of PD-L1 protein and the expression of PD-L1 and mRNA in nasopharyngeal carcinoma tissues in patients with TNM stage Ⅲ-Ⅳ, lymph node metastasis and history of smoking were higher than those in patients with TNM stage Ⅰ-Ⅱ,no lymph node metastasis, and no smoking history（P〈0.05）. Conclusion： The expression of PD-L1 protein and PD-L1 mRNA is highly expressed in nasopharyngeal carcinoma,and its expression is related to TNM staging,lymph node metastasis and smoking history.