有机相对魔芋葡甘聚糖/纳米羟基磷灰石/壳聚糖复合多孔骨组织工程支架材料性能的影响研究

Investigation of the organic phase impact on the property of KHC porous scaffold

以氨水为交联剂,冰乙酸为改性剂,生物相容性良好的可降解生物材料魔芋葡甘聚糖(KGM)、壳聚糖(CS)和纳米羟基磷灰石(纳米-HAP)为基本原料,在碱性溶液环境下将三者机械混合得到凝胶,经熟化、除碱和冷冻干燥等工艺手段,制备得到魔芋葡甘聚糖/纳米羟基磷灰石/壳聚糖(KHC)复合多孔支架。通过抗压强度和气孔率测试,以及X射线衍射、傅里叶红外光谱和扫描电镜等检测手段探讨KGM含量对复合支架材料结构与物化性能的影响,其后对实验较优组进行CS酸改性处理,并与未改性组进行对比分析。结果表明:当KGM含量为原比例(1KGM)时,支架材料有机相包裹无机相的能力最好,当KGM含量大于原比例(5/4KGM、3/2KGM)时,有机相包裹能力逐渐减弱;原比例组CS经酸改性后支架的包裹性能将进一步提升,酸改性组支架抗压强度最高,达到了1.1MPa。

Ammonia used as crosslinking agent,glacical acetic acid as modifier,and konjac glucomannan（KGM）/chistosan（CS）/nano-hydroxyapatite（nano-HAP）with good biocompatibility as raw materials,the gel with three components was prepared by magnetic stirring in the alkaline solution.Finally,the konjac glucomannan/nanohydroxyapatite/chitosan（KHC）composite scaffold was prepared by the steps of aging,alkaline removing and freezingdrying.The effect of KGM content on the physical and chemical property were investigated by compressive strength test,porosity test,XRD,FT-IR and SEM.The experimental group was also investigated between CS modified with acid and unmodified.The results indicated that when KGM content was in the level of 1 KGM,the organic phase of scaffold had the best ability to encapsulate the inorganic phase.When KGM content was higher than the original proportion（5/4 KGM,3/2 KGM）,the encapsulation capacity of organic phase decreased.It was also indicated that the ability of encapsulate could be further promoted by the acid modification,and the acid-modified group had the highest compressive strength,which can be reached to 1.1 MPa.