摘要
猪圆环病毒2型(PCV2)能引起断奶仔猪衰竭综合症,为了获得利于纯化的病毒和新型标记疫苗,采用病毒重组技术,选择利于纯化的6个氨基酸His标签作为标记表位,插入PCV2 Cap蛋白C端,拯救获得重组标记病毒rPCV2-His。间接免疫荧光和Western blot鉴定结果证明重组病毒所含的标签能稳定表达。通过镍柱纯化重组病毒,该病毒获得了纯化。纯化的重组病毒灭活后免疫小鼠,PCV2特异抗体显著提升,且能产生标记免疫抗体,小鼠攻毒显著降低体内PCV2含量。该重组病毒可作为PCV2新型疫苗研究重要材料。
Porcine circovirus type 2（ PCV2） can cause postweaning multisystemic wasting syndrome. In order to obtain the purified virus and develop novel marker vaccine against PCV2,a 6-amino-acid His tag was inserted into the C-terminus of capsid protein of PCV2. The recombinant mark virus rPCV2-His expressing the His tag was constructed and rescued. The recombinant marker virus was detected by indirect immunouorescence assay and Western blot,and was purified by nickel column. PCV2 specific antibody was significantly improved and His tag immune antibody was produced in mice inmunized with purified recombinant virus. PCV2 content was significantly decreased in mice inmunized with purified recombinant virus. The findings of this study may be used for the development of a novel PCV2 vaccine.
作者
华涛
唐波
常晨
刘国阳
张雪花
于漾
侯继波
张道华
HUA Tao1,2,3, TANG Bo1,2,3, CHANG Chen1,2,3, LIU Guoyang1,2,3,ZHANG Xuehua1,2,3, YU Yang1,2,3 , HOU jibo1,2,3, ZHANG Daohua1,2,3(1.Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Veterinary Immunology ＆ Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014; 2.National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014; 3.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Yangzhou 225009, Chin)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2018年第4期36-43,共8页
Journal of Agricultural Science and Technology
基金
江苏省农业自主创新项目[CX(14)5044]
农业部公益性行业(农业)科研专项经费项目(201303046)资助
关键词
PCV2
重组病毒
纯化
标记
免疫
PCV2
recombinant virus
purification
marker
immune
