代谢综合征“痰证”的形成及其兼杂特点与先天因素的关系

Relationship between the formation and characteristics of metabolic syndrome 'phlegm syndrome' and its congenital factors

目的:以代谢综合征(MS)患者为对象,研究MS痰证与MS相关基因SNP位点的关系,从遗传学的角度探讨MS痰证的形成与先天因素的关系。方法:以MS患者为对象,通过证素辨证方法结合Taq Man?SNP基因型检测技术,研究MS痰证与MS相关基因EXT2、MEM18、LIPG位点的关系,为揭示MS痰证的兼杂、影响脏腑的遗传学基础提供依据。结果:痰证组病位证素胆在EXT2风险基因型CC上的分布比例显著升高,与非胆比较,差异具有统计学意义(P<0.05)。在各基因型与病性证素的关系上,研究发现,病性证素气滞在EXT2风险基因型CC上的分布比例显著升高,与非气滞比较,差异具有统计学意义(P<0.05)。TMEM18基因rs6548238基因型其病性证素热在风险基因CC上比例升高,较非热比较,差异有统计学意义(P<0.05)。在LIPG基因rs4939883基因型上,非气滞基因CC比例明显高于气滞(P<0.05)。LIPG基因rs2156552基因型其病性证素气滞与非气滞比较,非气滞组在TT基因型上的比例明显高于气滞(P<0.05)。结论:EXT2基因风险基因型CC可能是MS痰证患者影响胆腑的遗传学基础之一,携带EXT2基因rs11037909基因型CC的人群可能更易兼夹气滞。携带TMEM18基因rs6548238基因型CC风险基因的人群可能更易兼夹病性证素热。

Objective： To study the relationship between metabolic syndrome （MS） phlegm syndrome and MS-related gene SNP loci by taking MS patients as objects, and observe the relationship between the formation of MS phlegm syndrome from the view point of genetics. Methods： The method of syndrome differentiation combined with TaqMan SNP genotype detection technology was used to study the relationship between MS phlegm syndrome and MS-related gene EXT2, MEM18, LIPG loci, and provide the evidence of MS phlegm syndrome characteristics and influence of viscera of genetic underpinnings. Results： in terms of relationship Of genotypes and symptoms, studies showed that disease location syndrome of gallbladder of EXT2 risk genotype CC remarkably increased compared with non-glallbladder group, the difference had statistical significance （P〈0.05）, Based on the relationship between genotypes and disease syndromes, it was found that the distribution of disease syndrome qi stagnation on EXT2 risk genotype CC was significantly higher than that of non qi stagnation, and the difference was statistically significant （P〈0.05）, For TMEM18 gane rs6548238 genotype, proportion of heat of risk genotype CC increased compared with non-fever group, the difference had statistical significance （P〈0.05）. For LIPG gene rs4939883 genotype, gene CC proportion of non- stagnation group was obviously higher than that of qi stagnation group, and the difference had statistical significance （P〈0.05）, For LIPG gene rs4939883 genotype, TT genotype proportion of non-stagnation group was obviously higher than that of qi stagnation group, and the difference had statistical significance （P〈0.05）. Conclusion： EXT2 gene risk genotype CC may be one of genetics basis on which MS phlegm syndrome patients affect gallbladder, People with EXT2 gene rs11037909 genotype CC are liable to suffering from qi stagnation, People with TMEM18 gene rs6548238 genotype CC risk gene are liable to suffering from heat.