YAP及P-YAP在RAW264.7细胞脓毒症模型中的表达

The research of YAP and P-YAP in sepsis model of raw264.7 cell

目的探讨YAP及P-YAP在RAW264.7细胞脓毒症模型中的表达。方法将对数生长期的RAW264.7细胞用内毒素(LPS)刺激RAW264.7细胞构建细胞脓毒症模型为实验组,未处理者为正常组。运用免疫荧光对YAP及P-YAP进行定位,Western blot检测YAP及P-YAP的表达情况。结果实验组YAP蛋白表达于胞核中,而P-YAP表达于胞质中。实验组YAP蛋白表达明显高于正常组(P<0.05),P-YAP蛋白表达明显低于正常组(P<0.05)。结论细胞脓毒症模型中YAP的高表达及P-YAP的低表达可能参与了脓毒症的发生。

Objective To investigate the expression of YAP and P-YAP in sepsis model of RAW264.7 cells. Methods RAW264.7 cells were stimulated by lipopolysaccharide （LPS） to establish the model of sepsis. The location of YAP and P-YAP was detected by immunofluorescence, and the expressions of YAP and P-YAP were detected by Western blot. Results In the sepsis model of RAW264.7 cells, the YAP protein was expressed in the nucleus, whereas the P-YAP was expressed in the cytoplasm. In the model of sepsis, the expression of YAP protein was significantly higher than that in the normal group, and the expression of P-YAP protein in the sepsis model was lower than that in the normal group. Conclusion High expression of YAP and low expression of P-YAP may be involved in the development of sepsis in sepsis model.