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L-苯丙氨酸聚肽的酶催化合成 被引量:1

Enzymatic Synthesis of L-Phenylalanine Polypeptide
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摘要 聚肽是由人工合成的氨基酸聚合物,具有良好的生物相容性和生物可降解性。本文以菠萝蛋白酶为催化剂,在温和条件下于非水体系中从L-苯丙氨酸甲酯聚合得到齐聚L-苯丙氨酸。通过FT-IR、~1HNMR、MS和MALDI-TOF确定了齐聚L-苯丙氨酸的结构和聚合度。通过单因素考察了菠萝蛋白酶催化聚合反应的最优条件。结果表明,在40℃下用2.44U菠萝蛋白酶于15%DMSO的磷酸缓冲液(pH=8.0)中催化0.43g L-苯丙氨酸甲酯盐酸盐聚合,反应3h后齐聚L-苯丙氨酸得率达到最大值,为35.67%。 Artificial polymers of amino acids,polypeptides,show the advantages of biocompatibility and biodegradability. In this study,the oligo-L-phenylalanine was prepared by the polymerization reaction of bromelain from L-phenylalanine methyl ester in the non-aqueous media under mild conditions. The structure and polymerization degree of the oligo-L-phenylalanine were elucidated by FT-IR,~1 H-NMR,MS and MALDI-TOF. The optimum polymerization conditions were evaluated through single-factor approach. The highest value of yield,35. 67%,could be achieved when 0. 43 g L-phenylalanine methyl ester hydrochloride in 15% DMSO phosphate buffer( pH = 8. 0) was polymerized by 2. 44 U bromelain at 40℃ for 3 hours.
作者 刘严华 黄登发 朱锦雯 崔正刚 王峰 Liu Yanhua, Huang Dengfa, Zhu Jinwen, Cui Zhenggang, Wang Feng(School of Chemical and Material Engineering, Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi 21412)
出处 《化学通报》 CAS CSCD 北大核心 2018年第4期361-365,383,共6页 Chemistry
关键词 L-苯丙氨酸甲酯盐酸盐 酶催化 聚合 表征 L-phenylalanine methyl ester hydrochloride Enzymatic Catalysis Polymerization Characterization
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