中间锦鸡儿CibHLH027基因的克隆和功能研究

Cloning and Functional Characterization of CibHLH027 Involved in Leaf Senescence from Caragana intermedia

叶片衰老是植物生长发育的最后阶段,黑暗、干旱和极端温度等会加速叶片衰老过程,严重影响植物的生长发育和作物产量。bHLH（basic helix-loop-helix）属于转录调控因子中的一个大家族,因具有保守性较高的bHLH结构域而得名,广泛存在于真核生物界。在中间锦鸡儿（Caragana intermedia）转录组数据库中得到一条候选bHLH基因全长序列,将其命名为Cib HLH027。正常和黑暗诱导的情况下,CibHLH027-OE株系较WT相比叶片衰老明显,并且黑暗处理后叶绿素含量下降、离子渗透率增大、细胞膜受损严重,说明CibHLH027参与了叶片衰老调控过程。

Leaf senescence is the last phase of plant development,environmental factors such as dark,drought,extreme temperature can promote leaf senescence,which may influence the plant growth and crop yield.Basic/helix-loop-helix（ bHLH） transcription factors（ TFs） belong to a superfamily of transcriptional regulators,it is named b HLH for its highly conserved b HLH domain,and widely exists in the eukaryotes. A candidate b HLH encoding gene,CibHLH027,was screened from the transcriptome library of Caragana intermedia. Compared with wild type,CibHLH027-OE promoted leaf senescence under both light and darkness,with reduced chlorophyll content,increased ion leakage and cell death under darkness,indicates that CibHLH027 is involved in leaf senescence.