精子DNA碎片指数与精液参数相关性的初步研究

A preliminary study on the correlation between human sperm DNA fragmentation index and semen parameters

目的研究精子DNA碎片指数(DNA fragmentation index,DFI)与精液参数的关系以及精子DFI在评价男性生育力方面的应用价值。方法收集3020例男性患者精液标本,采用吖啶橙染色配合流式细胞仪技术检测DFI,同时通过计算机辅助精液分析系统(CASA)检测精子浓度、前向运动精子百分率(PR,%)、精子总活力[PR+NP(非前向运动精子)%]、精子总数和精液量(用称量法测定精液体积)。结果精子浓度与DFI值无明显关系(r=0.003,P>0.05)。精液量和DFI值有明显关系(r=0.078,P<0.01),精子总活力和DFI值有明显关系(r=-0.447,P<0.01)。PR和DFI值有明显关系(r=-0.444,P<0.01),精子总数和DFI值有明显关系(r=0.069,P<0.01),差异均具有统计学意义。结论精子DNA碎片指数与精液量、精子总数存在明显正相关,与精子总活力、前向运动精子百分率呈明显负相关。在一定程度上反映了精液参数可以为评价精子DNA完整性提供参考。

Objective To investigate the correlation between human sperm DNA fragmentation index（DFI） and semen parameters, and evaluate the application value of DFI in male fertility. Methods Semen samples from 3020 male patients were collected. Sperm DNA fragmentation index（DFI） was detected by acridine orange staining and flow cytometry. Sperm concentration, total viability, progressive motility（PR） and the total number of sperm were calculated using computerassisted semen analysis system（CASA）. The semen volume was determined by weighing. Results There was no significant relationship between sperm concentration and DFI（r =0.003,P 〈0.05）. There was a significant correlation between semen volume and DFI（r = 0.078, P 〈0.01）, between the total sperm motility and DFI（r =-0.447, P 〈0.01）, between the progressive motility（PR） and DFI（r =-0.444, P 〈0.01）, between total number of sperm and DFI（r =0.069, P 〈0.01）. All the differences were statistically significant（P0.05）. Conclusion The sperm DNA fragment index was positively correlated with semen volume and total number of sperm, but it was negatively correlated with total motility and PR. These indicates that semen parameters may provide a reference for evaluating sperm DNA integrity.