共培养杜英叶片内生菌次级代谢产物研究

Secondary Metabolites from Endophytes of Elaeocarpus Decipiens Hemsl.with Co-cultivation Method

采用共培养方法在室温下以固体方式培养杜英内生菌,培养40d,以乙酸乙酯萃取3次,其浸提物经过正相硅胶柱层析,Sephadex LH-20,半制备液相等方法纯化获得7个次级代谢产物,其结构运用NMR,MS等光谱学方法鉴定,分别为daldinone A(1),alternariol(2),4-methylalternariol(3),alternuene(4),(E)-3-benzylidene-2-methylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione(5),(Z)-3-benzylidene-2-methylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione(6),甘油三醋酸酯(7).化合物1-7是首次从杜英的内生菌中获得,化合物1具有抗菌活性,化合物6首次从自然界中分离得到.

The endophtes from Elaeocarpus decipiens Hemsl. are cocultured for 40 days at the room tempera turewith the co culture method, and the ethyl acetate crude extract are treated with Silicon Gel, Sephadex I.H 20 and Semi preparative HPLC; and seven secondary metabolites are obtained; and then, their structures are elucidated as daldinone A（I） , alternariol（2） , 4 methyl alternariol（3）, alternuene（4） , （E） 3 benzylidene 2 methyl Hexahydropyrrolo[l ,2 a]pyrazine 1,4 dione（5）,（Z） 3 benzylidene 2 methylhexahydropyrrolo[l ,2 alpyra zine 1,4 dione（6） and glycerin triacetate（7） . Further, the compound 1 shows some antimicrobial ac tivity. The compounds 1 7 are firstly isolated from the endophytes of Elaeocarpus decipiens Hemsl., and the compound 6 is firstly isolated from the nature.