广州和厦门鼠类携带3种致腹泻病毒检测分析

Detection of three gastroenteritis-causing viruses in rodents captured in Guangzhou and Xiamen city

目的了解鼠类携带致腹泻病毒情况,充实感染性腹泻病原体动物宿主的流行病学背景资料。方法2012—2014年采用笼捕法捕捉鼠类,收集来自广东省广州市和福建省厦门市的鼠类粪便样品,提取病毒RNA,RT-PCR检测样品中的A组轮状病毒(RVA)、GI、GII型诺如病毒(No V)和人双埃可病毒(HPeV)的核酸序列,并对实验获得序列进行测序和系统进化树分析。结果共收集样品224份,分别来自188只褐家鼠、27只黄毛鼠、9只黄胸鼠。在褐家鼠粪便中检出1份HPeV核酸阳性,阳性率为0.53%(1/188),但病毒分型实验未成功。在所有标本中未发现RVA或GI、GII型NoV核酸阳性。结论首次发现在褐家鼠有携带HPeV的可能,但仍有待进一步研究证实。

Objective To examine the status of gastroenteritis-causing virus carriage in rodents and to provide epidemiological information on animal vectors of the pathogens.Methods We collected fecal samples from rodents captured in Guangzhou and Xiamen city between 2012 and 2014. Real-time reverse transcription PCR （RT-PCR） method was used to detect viral nuclear acids of norovirus （GI and GⅡ）, rotavirus group A, and human parechovirus. Subsequently, the nuclear acids of the identified viruses were sequenced and phylogenetically analyzed.Results A total of 224 fecal samples were collected from 188 Rattus norvegicus, 27 Rattus losea and 9 Rattus tanezumi. One sample （0.53%） from Rattus norvegicus was positive for human parechovirus. Unfortunately, it was failed to identify the genotype of the virus. There were no samples positive for norovirus or rotavirus group A.Conclusion It is the first report that Rattus norvegicus might carry human parechovirus. However further studies should be conducted to confirm this finding.