漆酶催化4-氨基苯酚聚合

Laccase-catalyzed Polymerization of 4-Aminophenol

在微型反应器（40℃）中,将漆酶加入醋酸缓冲溶液（pH=5.0）中催化4-氨基苯酚聚合,持续反应10 h后得到黑色粉末状聚合物。通过UV-Vis观察了4-氨基苯酚及其聚合物吸收光谱的变化,采用FTIR、1HNMR、MALDI-TOF MS、TGA和DSC对产物的结构和性能进行了表征。结果表明：所得聚合物在氯仿中不溶,在丙酮、四氢呋喃、N,N-二甲基甲酰胺和二甲基亚砜中均有良好的溶解性。UV-Vis、FTIR和1HNMR结果显示：聚合物中酚羟基保留,氨基参与反应,苯环上的氢原子部分参与反应,并且推测了聚合物的结构。MALDI-TOF MS、TGA和DSC结果显示：聚合物为低聚物,相对分子质量分布在300-1 300 Da,聚合度为3-12。聚4-氨基苯酚的分解温度为223℃,具有良好的耐热性能。

Laccase-catalyzed polymerization of 4-aminophenol was conducted in acetate buffer solution（pH = 5. 0） at 40 ℃. After continuous reaction for 10 h,black solid powers were obtained. UV-Vis spectra were used to observe the absorption spectra changes of 4-aminophenol and its polymer. The structure and properties of product were characterized by means of FTIR,1 HNMR,MALDI-TOF MS,TGA and DSC. The results showed that the as-prepared polymer was insoluble in chloroform,and soluble in acetone,tetrahydrofuran,N,N-dimethylformamide and dimethyl sulfoxide. UV-Vis,FTIR and1 HNMR spectra of product revealed that the synthetic polymer contained phenolic hydroxyl groups,the amine group of 4-aminophenol participated in the polymerization reaction,and partial hydrogen atoms on the benzene ring involved in the reaction. The possible structures of polymer were speculated. In addition,the obtained poly（4-aminophenol） was an oligomer with a molecular weight ranging from 300 to 1 300 Da,its degree of polymerization was 3 to 12. The polymer had excellent heat resistance with a decomposition temperature of 223 ℃.