摘要
为探究甜菜碱在参与西瓜抗枯萎病方面的作用,本研究以西瓜悬浮细胞为试验材料,利用镰刀菌酸诱导,测定细胞活性氧(ROS)的变化情况以及甜菜碱合成相关基因的表达变化等。结果表明:镰刀菌酸对西瓜细胞的生长有明显的抑制作用,通过外源添加甜菜碱可以缓解低浓度(≤100μmol/L)下镰刀菌酸的胁迫;通过镰刀菌酸诱导西瓜细胞,可以使细胞内ROS迅速升高,并在30 min达到最大值,而同时添加甜菜碱处理可以明显降低细胞ROS含量;此外,镰刀菌酸诱导可以使甜菜碱合成基因Cl BADH和Cl CMO基因表达量呈现先上升后缓慢下降的趋势,且0.5 h相对表达量达到最高,这证明镰刀菌酸对西瓜甜菜碱合成基因Cl BADH和Cl COM的表达有不同程度诱导作用。上述研究结果初步表明甜菜碱在参与西瓜抗枯萎病方面具有一定作用,为加强对西瓜枯萎病抗病机制的探索和发掘抗性相关基因提供了理论支持。
To explore the effectivity of betaine against Fusarium wilt in watermelon, the change of reactive oxygen species (ROS) and the expression of betaine biosynthesis genes were determined after the suspension cells of watermelon elicited by fusaric acid. The results showed that the fusaric acid had significant inhibition on the growth of watermelon cells, but exogenous betaine could alleviate the stress accordingly. Elicition watermelon cells by Fusarium acid could rapidly raise the ROS content and ROS content culminated at 30 min. Whereas the exogenous betaine could decrease the ROS obviously. Gene expression of both ClBADH and CICMO after inducted by fusaric acid increased and the expression level reached the maximum at 0.5 h which indicated that betaine could be induced against fusaric acid. The above results showed that betaine might be involved in the resistance reinforcement against Fusarium wilt. The study would lay a foundation for further research and meanwhile provide a theoretical support for exploring the mechanism of resistance and foridentificating resistance related genes against Fusarium wilt in watermelon.
作者
徐子健
孙梦利
江雪飞
乔飞
XU Zijian1, SUN Mengli1, JIANG Xuefei1, QIAO Fei2(1 Institute of Tropical Agriculture and Forestry, Hainan University, Hainon Key Laboratory of Sustainable Utilization of Tropical Biological Resources(Ministry of Education, National Key Laboratory Breeding Base ), Haikou, Hainan 570228, China 2 Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences. Danzhou Hainan 571737,China)
出处
《热带作物学报》
CSCD
北大核心
2018年第2期355-360,共6页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31760595)
海南省教育厅高等学校科学研究项目(No.Hnky2017-6)
海口市重点科技计划项目(No.2017045)
中国热带农业科学院基本科研业务费专项资金(No.1630052016006).