补肾健骨中药对去势后大鼠骨质疏松症激素水平表达和骨密度影响的研究

Effect of Tonifying Kidney and Strengthening bone Chinese Herbs on the Expression of Hormone Level and bone Mineral Density in Ovariectomized Rats

目的:探究对去势后大鼠应用补肾健骨中药治疗骨质疏松症的疗效与作用机制。方法:研究用大鼠为Wister普通级大鼠,造模方法为将大鼠进行去卵巢处理来复制大鼠绝经后骨质疏松的实验模型,对实验大鼠进行随机分组:补肾健骨中药组、假手术组、雌二醇组、仙灵骨葆胶囊组、模型空白五组。补肾健骨中成药:仙灵骨葆胶囊。补肾健骨中药方剂治疗15周进行预防,阳性对照组使用尼尔雌醇、仙灵骨葆胶囊进行治疗,实验对照组设为模型空白对照组、假手术组。大鼠血清中促黄体生成素(LH)、骨钙素(BGP)、促卵泡激素(FSH)、雌激素(E2)水平检测运用放免方法检测;并检测大鼠骨密度(BMD),应用双能X线骨密度仪并取大鼠股骨与胫骨部位检测。结果:实验大鼠血清学指标结果比较:治疗后与空白对照组进行比较,补肾健骨中药组的E2的水平显著提高(P<0.05),而BGP水平显著下降(P<0.05),而激素FSH、LH水平均下降。实验大鼠经治疗后骨密度结果比较:治疗后与空白对照组进行比较,补肾健骨中药组变化差异最大(P<0.05)。结论:补肾健骨中药治疗绝经后骨质疏松效果明显,可有效预防绝经后骨质疏松的发生。

Objective：To investigate the curative effect and mechanism of kidney-reinforcing and reinforcing-reinforcing herbs on osteoporosis in ovariectomized rats.Methods：The rats were used as normal Wister rats. The model was made by ovariectomized rats to replicate the experimental model of postmenopausal osteoporosis in rats.The rats were randomly divided into five groups： Sham operation group, estradiol group, xianling Gubao capsule group and model blank five groups.Kidney bone medicine：Xianling Gubao capsule .Kidney strengthening bone Chinese medicine prescription for 15 weeks of prevention, the positive control group using nilestriol, Xianling Gubao capsule for treatment, the experimental control group was set as the model blank control group, sham operation group.The levels of serum LH, BGP, FSH and E2 were detected by radioimmunoassay （ RIA ） .The bone mineral density （ BMD ） Can X-ray absorptiometry and take the rat femur and tibia parts of the test.Results： Compared with the blank control group, the level of E2 in Bushenji- angyu Herbal Medicine group was significantly increased （ P〈0.05 ）, while the BGP level was significantly decreased （ P〈0.05 ）, while the levels of hormone FSH, LH levels decreased.Compared with the control group, the difference of BMD between experimental rats after treatment was significant （ P〈0.05 ） .Conclusion：The traditional Chinese medicine of strengthening the kidney and strengthening the bone is effective in treating postmenopausal osteoporosis, which can effectively prevent the occurrence of postmenopausal osteoporosis.