杉寄生中黄酮类成分薄层色谱鉴别研究

Identification of Flavonoids in Macrosolen cochinchinensis( Lour.) Van Tiegh. by TLC

目的建立杉寄生中黄酮类化合物的薄层色谱(TLC)鉴定方法,为药材的质量控制提供依据。方法以特征成分落新妇苷和芦丁为指标,采用硅胶G高效薄层板,以乙酸丁酯-甲醇-水-甲酸(7∶1.5∶1∶1.5,V/V)为展开系统,采用薄层色谱法对不同采集地的杉寄生药材进行定性鉴别。结果样品的薄层色谱图斑点清晰,分离度良好,但不同产地的杉寄生薄层色谱结果存在差异。结论本研究建立的方法薄层行为良好,且简便易行,适用于杉寄生的定性鉴别。

OBJECTIVE To establish a new qualitative method for the identification of flavonoids in Macrosolen cochinchinensis（ Lour.） Van Tiegh. by thin-layer chromatography（ TLC）,and it provided the basis for the quality control of medicinal materials.METHODS Astilbin and rutin were used as indicators,with adopt silica gel G254 thin layer board,butyl acetate-methanol-H2 Oformic acid（ 7 ∶1.5 ∶1 ∶1.5,V/V） as developing agent. Macrosolenco chinchinensis（ Lour.） Van Tiegh. from different areas were identified by TLC. RESULTS There were differences in TLC of samples from different areas. CONCLUSION The TLC method is effective for the identification of Macrosolen cochinchinensis（ Lour.） Van Tiegh.,which is simple and easy to operate.