过表达含Ⅰ型血小板结合蛋白基序的去整合素样金属蛋白酶18基因抑制人骨肉瘤细胞的增殖与迁移被引量：3

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摘要目的探讨含Ⅰ型血小板结合蛋白基序的去整合素样金属蛋白酶(ADAMTS)18基因对人骨肉瘤细胞增殖与迁移作用及其机制。方法将ADAMTS18过表达载体和空载体分别转染143B和U-20S人骨肉瘤细胞,细胞计数试剂盒(CCK)-8和Transwell小室实验研究过表达ADAMTS18基因对143B和U-20S细胞增殖和迁移作用,蛋白质Western印迹实验检测ADAMTS18和蛋白激酶B(AKT)蛋白表达水平。结果与空载体转染细胞株比较,过表达ADAMTS18基因可显著抑制143B和U-20S细胞增殖和迁移能力,并下调143B和U-20S细胞AKT蛋白水平。结论 ADAMTS18经下调AKT通路活性而抑制人骨肉瘤细胞恶性表型。

作者柴瑛

机构地区佳木斯大学附属第一医院骨科

出处《中国老年学杂志》 CAS 北大核心 2018年第7期1705-1707,共3页 Chinese Journal of Gerontology

基金黑龙江省自然科学基金项目(No.D200966) 佳木斯大学研究生科技创新项目(YM2016-006)

关键词骨肉瘤含Ⅰ型血小板结合蛋白基序的去整合素样金属蛋白酶(ADAMTS)18 蛋白激酶B(AKT)

分类号 R738.1 [医药卫生—肿瘤]

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1徐红英,肖茜,范宇,穆俊豪,邵辨非,任国胜.ADAMTS18基因过表达可抑制人乳腺癌细胞的迁移与侵袭[J].肿瘤,2016,36(7):749-757. 被引量：3

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