用流式细胞光度术研究PGE_2对小鼠骨髓CFU-GM细胞周期的影响

A STUDY OF THE EFFECT OF PGE_2 ON CFU-GM CELL CYCLE IN MOUSE BONE MARROW WITH FLOW CYTOMETRY

在一定PGE_2浓度(4.8×10^(-9)mol/L)作用下,小鼠骨髓细胞CFU-GM经4.5d和7d培养后,其增殖状态下的细胞G_n/G_r期细胞数均比对照组增加,S期细胞数减少,G_2+M期细胞数也有下降,但不明显。在不同PGE_2浓度(2.8×10^(-9)～2.8×10^(-6)mol/L)作用下,经4d培养,随着PGE_2浓度增加,G_n/G_1期细胞数递增,而S期细胞数却随PGE_2浓度增加而减少,G_2+M细胞数也减少,但与PGE_2剂量关系不明显。以上实验结果提示,PGE_2主要抑制G_1期细胞向S期细胞的转化,阻断S期细胞生长。 此外,通过流式细胞光度术(FCM)对小鼠骨髓细胞CFU-GM集落细胞的前向角和90°散射光测定,与对照组比较,前者无变化,后者变化较明显。此结果表明,PGE_2对细胞内部颗粒的折光度有影响。

The effects of prostaglandin E2 (PGE2) on the CFU-GM cell cycle of mouse bone marrow in vitro was evaluated. After exposure to certain dose (4.8 × 10-8mol/L) of PGE2, the percentage of G0/G1 phase cells in mouse bone marrow increased and S phase cells decreased, while G2+M phase cell showed no significant change after culturing for different times (4.5 and 7 days). The results suggested that the transition of GO/G! to S phase of CFU-GM cells in mouse bone marrow was inhibited by PGE2 and the blocking point was at S phase. The blocking effect was dose dependent within the range of 2.8×10-9-2.8 × 10-6mol/L. The forward and ninety-degree light scattering measurement has been widely used for estimation of cell size and shape and was performed for CFU-GM cells in mouse bone marrow in persent study. The peak of ninety-degree light scattering of CFU-MG cells treated with PGE2 shifted to right as compared with the control, while forward light scattering showed no change, indicating that the probable change was in the intracellular structure.