miR-130a靶向调节SMAD4基因对卵巢癌SKOV3细胞增殖和侵袭的影响

Effects of miR-130a targeting SMAD4 gene on the proliferation and invasion of ovarian cancer SKOV3 cells

目的探讨miR-130a对卵巢癌SKOV3细胞增殖及侵袭能力的影响。方法将卵巢癌SKOV3细胞分为miR-130a抑制剂组、miR-130a模拟物组和对照组,分别将miR-130a抑制剂或miR-130a模拟物转染至miR-130a抑制剂组或miR-130a模拟物组细胞,对照组细胞转染无关序列,Real-time PCR法检测各组细胞miR-130a表达水平,分别用MTT方法与Transwell细胞侵袭实验检测转染后各组SKOV3细胞的增殖与侵袭能力变化。Real-time PCR与Western blot实验分别检测各组细胞SMAD4 mRNA和蛋白表达水平。结果转染miR-130a模拟物后,SKOV3细胞miR-130a的表达水平显著升高,SKOV3细胞的增殖与侵袭能力显著降低,SMAD4 mRNA及蛋白的表达水平显著降低(P<0.01);转染miR-130a抑制剂后,SKOV3细胞miR-130a的表达水平显著降低,SKOV3细胞的增殖与侵袭能力显著升高,SMAD4 mRNA及蛋白的表达水平显著升高(P<0.01)。结论 miR-130a抑制卵巢癌SKOV3细胞的增殖和侵袭可能与下调SMAD4的表达相关。

Objective To investigate the effect of miR-130a on the proliferation and invasion of ovarian cancer SKOV3 cells. Methods The ovarian cancer SKOV3 ceils were divided into miR-130a inhibitor group, miR-130a mimics group and control group, and then, miR-130a inhibitors or miR-130a mimics were transfeeted into SKOV3 cells of miR- 130a inbibitor group or miR-130a mimics group respectively, control group was transfected with unrelated sequence. The miR-130a level was detected by real-time PCR, the proliferation and invasion ability of SKOV3 cells were detected by MTT method and transwell cell invasion assay after transfection in every＂ group. Real-time PCR and Western blot methods were used to detect the expressions of SMAD4 mRNA and protein. Results After miR-130a mimics was transfected, the expression level of miR-130a in SKOV3 ceils was decreased significantly, the proliferation and invasion capacity of SKOV3 cells was decreased significantly, the expression level of SMAD4 mRNA and protein was significantly reduced （ P〈0.01 ） . After miR-130a inhibitors was transfected, the expression level of miR-130a in SKOV3 cells was increased, the proliferation and invasion capacity of SKOV3 cells was increased, the expression level of SMAD4 mRNA and protein was significantly increased （ P〈0.O1 ） . Conclusion The inhibition of miR-130a on the proliferation and invasion of ovarian cancer SKOV3 cells might be related to down-regulating the expression of SMAD4.