电针结合康复训练对缺血脑卒中大鼠Notch1与Notch4表达的影响

Effects and possible mechanism of electroacupuncture combined with rehabilitation training on the neural function of rats with ischemic stroke

目的探讨电针结合康复训练治疗对脑卒中大鼠神经功能及大鼠大脑皮层Notch1与Notch4表达的影响。方法 60只SD大鼠随机分为3组:假手术组(S)、模型组(M)与电针+康复训练组(E),采用大脑中动脉阻塞法(MCAO)建立局灶性脑缺血动物模型。采用Longa法评定实验大鼠的神经功能缺损程度,免疫组织化学方法与Western blot和real time PCR方法检测大鼠大脑皮层Notch1与Notch4表达。结果与模型组相比,电针+康复训练组大鼠在实施治疗后,Longa评分明显改善(P<0.01)。与假手术组相比,模型组大鼠大脑皮层Notch1与Notch4蛋白与mRNA表达水平显著升高(P<0.01);而与模型组相比,电针+康复训练组大鼠大脑皮层Notch1与Notch4蛋白与mRNA表达水平升高更为显著(P<0.01)。结论电针结合康复训练改善脑卒中大鼠的神经功能可与上调大鼠大脑皮层Notch1与Notch4的表达相关。

Objective To investigate the effects of electroaeupuncture combined with rehabilitation training on the neural function of rats with ischemic stroke and the expression of Notchl and Notch4 in the cerebral cortex of rats. Methods 60 SD rats were randomly divided into 3 groups： sham operation group（S）, model group（M）and electroaeupuncture plus＋rehabilitation training group（E）, and middle cerebral artery ocelusion（MCAO） was used to establish focal cerebral ischemia animal model. The degree of neurological deficit was evaluated by Longa method, the expression of Notch4 and Notehl in the cerebral cortex was detected by immunohistochemistry and Western blot, and real time PCR technology. Results Compared with the model group, the Longa score of the rats in electroacupuncture＋rehabilitation training group were improved significantly after the treatment（P〈O.01）. The expression levels of Notchl and Notch4 proteins and mRNAs in cerebral cortex were increased significantly in model group than in sham operation group, and increased significantly in eleetroaeupuncture＋rehabilitation training group than in model group（P〈0.01）. Conclusion The improvement of electroacupuneture ＋rehabilitation training on the neural function of cerebral apoplexy ratsmight be related to the upregulation of Notehl and Notch4 expression in the cerebral cortex.