多肽pATMtide在先天性心脏病中的作用

Effect of pATMtide on congenital heart disease

目的:研究多肽pATMtide在P19细胞(小鼠畸胎瘤细胞)的分化及斑马鱼心脏发育中的作用,探讨其与先天性心脏病(congenital heart disease,CHD)之间的关系。方法:从已报道的CHD羊水多肽图谱中筛选出1条表达明显上调的多肽pATMtide。在体外实验中,通过加入外源性多肽pATMtide,检测其对P19细胞分化的影响;在体内实验中,将多肽pATMtide通过显微注射技术注入到斑马鱼受精卵,观察其对斑马鱼心脏发育的影响。结果:与对照组相比,加入外源性多肽pATMtide明显抑制了P19细胞的分化,心脏发育各相关标志物表达水平明显下调。斑马鱼实验表明:多肽pATMtide可造成斑马鱼心脏发育畸形。结论:多肽pATMtide可影响P19细胞分化并导致斑马鱼心脏发育畸形,表明多肽pATMtide与CHD的形成有一定的关系。

Objective： To investigate the function of pATMtide in the heart development of zebra fish and differentiation of P19 cells. Methods： An obvious up-regulated polypeptide pATMtide was found from our previous study. In vitro experiment, the effect of pATMtide on P19 cells differentiation were detected; and in vivo experiment, after microinjecting the zygotes zebra fish with pATMtide, the cardiac development of zebra fish was observed. Results： The differentiation and related cardiac biomarkers of P19 cells are reduced as well as the heart development of zebrafish after adding pATMtide. Conclusion： The differentiation and development are influenced after adding pATMtide. It indicates that pATMtide may play an important role in congenital heart disease.