TRIM24在乳腺癌组织中的表达与患者淋巴节转移的相关性

Correlation of TRIM24 expression with lymph node metastasis in breast carcinoma

目的:分析TRIM24在乳腺癌中的表达水平。方法:通过oncomine在线数据库分析TRIM24基因在乳腺癌组织及正常组织中的表达谱,聚类分析;选择复旦大学附属华山医院普外科收治的108例乳腺癌患者的组织标本,用免疫组织化学及免疫荧光检测TRIM24蛋白的表达及分布,用Western印迹检测TRIM24蛋白的表达。分析TRIM24的阳性表达与患者淋巴结转移的相关性。结果:癌症基因组图谱(TCGA)等数据库中显示TRIM24在乳腺癌组织中高表达。TRIM24蛋白在乳腺癌组织标本中的表达也显著高于癌旁组织,在细胞核和细胞质中均有表达。TRIM24的阳性表达与患者的淋巴结转移状态呈正相关(P<0.05)。结论:TRIM24在乳腺癌组织中高表达,高表达TRIM24的患者更易发生淋巴结转移。

Objective： To analyze the expression level of TRIM24 in breast cancer. Methods： By using oncomine online database, expression of TRIM24 gene in breast cancer tissues was analyzed by cluster analysis. In 108 cases of patients from Department of General Surgery, Huashan Hospital Affiliated to Fudan University, the expression and distribution of TRIM24 protein was detected by immunohistochemistry and immunofluorescence. The expression of TRIM24 protein was also detected by Western blot. The correlation between positive expression of TRIM24 and lymph node metastasis in patients was analyzed. Results： The online database including Cancer Genome Atlas（TCGA） showed high expression of TRIM24 in breast cancer tissues, and the expression of TRIM24 protein in breast cancer tissues was significantly higher than that in paracancerous tissues. TRIM24 protein was expressed both in nucleus and cytoplasm. The positive expression of TRIM24 was positively correlated with lymph node metastasis（P〈0.05）. Conclusion： High expression of TRIM24 in breast cancer indicates that it is closely related to lymph node metastasis in breast cancer.