3D打印丝素蛋白-Ⅱ型胶原软骨支架

Silk fibroin-type Ⅱ collagen cartilage scaffold fabricated by 3D printing technology

运用三维制图软件Solidworks设计了软骨支架宏观结构,采用3D打印技术和冷冻干燥技术制备了丝素蛋白-Ⅱ型胶原软骨支架。通过实验测试了支架的密度、孔隙率和弹性模量;在支架上接种软骨细胞后,采用MTT法、HE染色和扫描电镜观察3种方法分析了细胞在支架上的增殖和形态。结果显示,丝素蛋白-Ⅱ型胶原软骨支架弹性模量具有率相关性,即随着应变率增加,支架的弹性模量增大;支架的密度和孔隙率分别为(0.086 6±0.008 4)g/cm3和(89.3±3.26)%。支架接种细胞培养7 d后,细胞生长增殖加快;HE染色观察发现,细胞在表层区生长最多,深层区最少;扫描电镜观察发现,支架孔径形状规则,通透性较好,细胞多分布于孔壁表面。

The macroscopic structure of cartilage scaffold was designed by using Solidworks,and the silk fibroin-type Ⅱ collagen cartilage scaffold was prepared by 3 D printing technique and freeze-drying technique. The density,porosity and elastic modulus of the scaffolds were tested by experiments. The proliferation of the cells was analyzed by MTT assay,HE staining and scanning electron microscopy. The results showthat the silk fibroin-type II collagen scaffold is dependent on the strain rate. The elastic modulus of scaffold increases with the increase of strain rate. The density and porosity of scaffold were（0. 086 6 ± 0. 008 4） g/cm3 and（89. 3 ± 3. 26）%,respectively. The cell growth and proliferation were accelerated after 7 days of inoculation. By analyzing the results of HE staining,it is found that the cells growmost in the surface area and there are the least cells in the deep region. The microscopic images by Scanning electron microscopy（SEM） reveal that the diameter of scaffold is regular and the permeability is better. The cells are mostly distributed on the surface of the spine.