紧密连接蛋白ZO-1在肾癌中的表达及其临床意义

Expression and clinical significance of tight junction associated protein ZO - 1 in patients with renal cell carcinoma

目的 探讨闭锁小带蛋白（sonula occluden-1,ZO-1）这种紧密连接相关蛋白在肾癌中的表达情况及其临床意义。方法 采用免疫组织化学法检测55例肾癌患者手术切除的肾脏病理标本中正常肾组织以及肾癌组织中ZO-1蛋白的表达，并分析肾癌组织中ZO-1的表达与肿瘤的侵袭和转移之间的关系。结果 ZO-1蛋白在正常肾组织和肾癌组织中的阳性表达率分别为74.5%和32.7%。 两组间进行比较显示，ZO-1蛋白在肾癌组织中的表达水平明显低于正常肾组织，组间具有差异性（P〈0.05）。在肾癌组织中，ZO-1蛋白的表达水平与癌组织的临床分期、分化程度以及淋巴结转移有关。结论 人肾癌组织中ZO-1蛋白的表达较正常肾组织减少，并且其可能参与肾癌肿瘤细胞的侵袭和转移。

Objective To investigate the expression of tight junction associated protein ZO-1 in renal cell cancer and its clinical significance. Methods Immunohisochemistry was used to detect the expression of ZO-1 protein in normal renal tissue and renal cell carcinoma tissue, and all the tissues were derived from the pathological specimens of 55 cases of renal cell carcinoma patients. The correlation between the expression of ZO-1 and the invasion and metastasis of renal cell carcinoma was analyzed. Results The positive expression rate of ZO-1 protein in normal renal tissue and renal cell carcinoma was 74.5% and 32.7%, respectively. The expression level of ZO-1 in renal cell carcinoma tissue was significantly lower than that in normal renal tissue, so there was a difference between two groups. In renal cell carcinoma tissue, the expression level of ZO-1 was involved with the clinical stage, degree of differentiation and lymph node metastasis of carcinoma. Conclusions The expression of ZO-1 in human renal cell carcinoma tissue is down-regulated compared with normal human renal tissue, and it may be involved in the invasion and metastasis of renal carcinoma.