五肽胃泌素和胆囊收缩素对血管灌流大鼠离体胃运动的影响

EFFECTS OF PENTAGASTRIN AND CHOLECYSTOKININ ON GASTRIC MOTILITY IN ISOLATED VASCULARLY PERFUSED RAT STOMACH

用血管灌流大鼠离体胃制备,研究五肽胃泌素(G5)和八肽胆囊收缩素(CCK8)对胃窦收缩运动的影响。结果表明:(1)血管灌流G5和CCK8都能显著兴奋胃窦收缩运动,并有量效关系;(2)抗胃泌素血清(1:100)可完全取消G5对胃窦收缩运动的兴奋作用;(3)CCK受体阻断剂双丁酰环磷鸟苷和抗CCK8血清(1:100)都能完全取消CCK8对胃窦收缩运动的兴奋作用;(4)M受体阻断剂阿托品能完全阻断G5对胃窦收缩运动的兴奋作用,部分阻断CCK8对胃窦收缩运动的兴奋作用。上述结果提示:(1)G5可特异性兴奋血管灌流大鼠胃窦收缩运动,该作用通过壁内胆碱能神经系统介导;(2)CCK8对血管灌流大鼠胃窦收缩运动亦有特异性兴奋作用,该作用只是部分与壁内胆碱能神经系统有关。

Effects of pentagastrin(GS) and octa-cholecystokinin (CCK8) on gastric motility were studied on the preparation of isolated vascularly perfused rat stomach. The results showed: (l)vascular perfusion of G5 and CGK8 could markedly stimulate antral contraction and have dose-effect relationship; (2) antigastrin serum could block the stimulatory effect of G5 on gastric motility completely; (3) anti-CCK8 serum and CCK receptor antagonist Bt2cGMP could also markedly block the stimulatory effect of CCK8 on gastric motility; (4) M receptor antagonist atropine could block the stimulatory effects on gastric motility by G5 completely and CCK8 partly. The results indicated that G5 could stimulate isolated vascularly perfused rat stomach motility specifically through mediation by cholinergic nervous system. CCK8 could also stimulate isolated vascularly perfused rat stomach motilty specifically, but the stimulation was only partly mediated by cholinergic nervous system.