凤仙花嫁接后代的镉积累特性

Cadmium Accumulation Characteristics of Post-grafting Generation of Impatiens balsamina

以凤仙花为试材,采用未嫁接、同株嫁接、异株同苗嫁接、异株异苗嫁接方法对其进行了嫁接处理,通过盆栽试验在镉污染土壤条件下,研究了不同嫁接方法对凤仙花后代镉积累的影响。结果表明:嫁接处理提高了凤仙花后代的生物量、光合色素(叶绿素a、叶绿素b、叶绿素总量和类胡萝卜素)含量、抗氧化酶(SOD、POD和CAT)活性,促进了其在镉污染条件下的生长;提高了凤仙花后代的镉含量、转运系数、镉积累量和转运量系数,这些指标的大小顺序均为异株异苗嫁接后代>异株同苗嫁接后代>同株嫁接后代>未嫁接后代。与未嫁接后代相比,同株嫁接、异株同苗嫁接后代和异株异苗嫁接后代的凤仙花根系镉积累量分别增加了34.13%、63.49%和89.22%,地上部镉积累量分别增加了55.39%、105.92%和171.58%。因此,采用自根苗嫁接能够促进镉污染条件下凤仙花后代的生长,提高其对镉的吸收与积累,从而提高了其对镉污染土壤的修复能力,其中异株异苗嫁接后代的效果最佳。

Impatiens baZsamina was conducted to study the effects of non-grafted, self-rooted grafting by the same plant seedling （SG）, self-rooted grafting by two uniform plant seedlings （UG）, and self- rooted grafting by two different development stages of plant seedlings （DG） on cadmium （Cd） accumulation of post-grafting generation of Impatiens balsamina under Cd contaminated condition by pot experiment. The results showed that grafting all increased the biomass, photosynthetic pigments （chlorophyll a,chlorophyll b, chlorophyll and carotenoid） contents, antioxidant enzymes （SOD, POD and CAT） activities of post-grafting generation of I. balsamina,indicating that grafting could increase the resistance of generation of I. balsamina to Cd, and promote their growth under Cd contaminated condition. Grafting also increased the Cd content, translocation factor, Cd accumulation amount and translocation amount factor of post-grafting generation of I. balsamina, and the order of these items all were ranked as DG〉UG〉SG〉non-grafted treantment. Compared with un-grafted treantment, the Cd accumulation in roots of SG, UG and DG increased by 34. 13%, 63.49% and 89.220%, respectively, and in shoots increased by 55.39%, 105.92% and 171.58%, respectively. Therefore, self-rooted grafting could promote the growth and improve the Cd absorption and accumulation of generation of I. balsamina under Cd contaminated condition, which improved the remedy ability of I. balsamina to Cd contaminated soil, and DG was the best one.