姜黄素预处理对沙漠干热环境猪腹部枪弹伤模型生存时间的影响

Effects of Curcumin Pretreatment on the Survival Time of Porcine with Abdomenal Gunshot Injury in Dry-heat Environment of Desert

目的:探讨姜黄素预处理对沙漠干热环境腹部枪弹伤动物模型生存时间的影响。方法:将24头长白仔猪随机分为2组(n=12):姜黄素预处理组和对照组。姜黄素预处理组给予长白仔猪姜黄素100 mg/kg拌料饲养,每天1次,连续7天,对照组给予常规饲料喂养。第8天,将两组猪放入西北特殊环境人工实验舱内,设置环境温度(40.5±0.5)℃,相对湿度(10±2)%,禁食水,放置3小时后建立腹部枪弹伤模型,模型成功后继续放在沙漠干热环境中,每10分钟检测体温变化,并计算长白仔猪的存活时间。结果:姜黄素预处理组平均存活时间为(85.27±2.39)min,对照组的动物模型平均生存时间为(60.10±3.25)min,姜黄素预处理组的生存时间平均比对照组延长约25 min,两组的Kaplan-Meier生存曲线经Log Rank检验具有显著性差异(P<0.01)。建立腹部枪弹伤模型后,在相应时间点,姜黄素预处理组的体温明显低于未处理组(P<0.01),姜黄素预处理组70 min时的体温与对照组50 min体温比较差异无统计学意义(P>0.05)。结论:姜黄素预处理可明显提高干热环境下猪腹部枪弹伤模型的生存时间,体温升高可能是反映干热环境腹部枪弹伤后病程进展的重要指标。

Objective： To explore the effects ofcurcumin pretreatment on the survival time of porcine with abdomenal gunshot in- jury in dry-heat environment of desert. Methods： 24 Changbai piglets were randomly divided into two groups （n=12）： curcumin pretreat- ment gurop and control group. The curcumin pretreatment group was given a dose of curcumin with the concentration 100 mg/kg once a day in diet for 7 consecutive days, the control group was given normal diet. On the 8th day, both groups were transferred to the climate cabin （The Simulated Climate Cabin for Special Environment of Northwest of China） with the conditions of （40.5±0.5）℃ temperature, （10± 2） % relative humidity, food and water were prohibited, after being exposed in the dry-heat environment for 3 h, the abdomenal gun- shot injury model were established for all the piglets, then the animal models were still placed in the dry-heat invironment, the survival time was calculated and the temperature was taken at corresponding time point. Results： The mean survival time of curcumin pre- treatmne group and control group were （85.27± 2.39）rain and （60.10± 3.25）min, the curcumin group survived about 25rain longer than that of the control group, Kaplan-Meier survival curve showed significant difference tested by Log Rank method（P〈0.01）. After establishment of the gunshot model, the temperature were lower in the curcumin pretreatment group than that of the control group at correspond- ing time point（P〈0.01）, the temperature at 70min had no significant difference compared with the temperature at 50min（P〉0.05）. Conclu- sions： Curcumin preteatment could evidently prolong the survival time of porcine abdomenal gtmshot model, and the rise of temperature may be important indicators reflecting the pathophysiological progression of the abdomenal gunshot injury in the dry-heat environment of desert.