磷酸胆碱胞苷酰转移酶α在口腔鳞状细胞癌中的表达及潜在临床意义

Expression and potential clinical significance of cytidine triphosphate： phosphocholine cytidylyltransferase-α in oral squamous cell carcinoma

目的探讨磷酸胆碱胞苷酰转移酶α（cytidine triphosphate：phosphocholine cytidylyltransferase-α，CCT-α）在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中的表达及潜在临床意义。 方法以2016年5月至2016年7月在中南大学湘雅二医院口腔中心收集的58例OSCC组织标本为实验组，同一患者的癌旁组织为对照组。免疫组织化学法检测CCT-α在OSCC组织及癌旁组织中的表达情况，结合患者的临床病理资料分析CCT-α与OSCC患者肿瘤的临床病理特征的关系。采用实时荧光定量PCR及蛋白质印迹法检测OSCC细胞株和正常口腔上皮细胞株中CCT-α mRNA及CCT-α蛋白的表达情况。 结果免疫组化染色结果示CCT-α阳性染色位于细胞核，CCT-α在OSCC组中的表达显著高于癌旁组（P=0.000）。临床病理资料分析示OSCC组织中CCT-α的表达水平与患者吸烟、饮酒史（P=0.001，P=0.004）及肿瘤大小（P=0.005）、分化程度（P=0.000）、是否发生淋巴结转移（P=0.000）密切相关。有吸烟、饮酒史的患者，CCT-α蛋白的表达水平明显升高。实时荧光定量PCR结果显示CCT-α mRNA在OSCC细胞株中的表达显著高于口腔上皮细胞株（P=0.016）。蛋白质印迹法结果显示CCT-α蛋白在OSCC细胞株中的表达显著高于口腔上皮细胞株，其蛋白表达趋势与CCT-α mRNA一致。 结论CCT-α可能在OSCC的发生发展中有重要作用。

ObjectiveTo examine the expression and potential clinical significance of CCT （cytidine triphosphate： phosphocholine cytidylyltransferase）-α in oral squamous cell carcinoma （OSCC）. MethodsFifty-eight OSCC and paired adjacent non-malignant epithelia samples （between May 2016 and July 2016） were obtained from dental center, Second Xiangya Hospital, Central South University. CCT-α expression was examined by immunohistochemistry. The relationship between CCT-α and clinicopathological features of OSCC patients was analyzed. Quantitative real-time PCR and Western blot were performed to measure the expression of CCT-α mRNA and protein level in several OSCC cell line and two normal oral epithelial cell line. ResultsImmunohistochemistry showed that CCT-α positive staining was found in cell nuclear of OSCC cells and adjacent epithelial cells. CCT-α was positively expressed in OSCC, which was significantly higher than that adjacent to carcinoma tissues （P=0.000）. The expression of CCT-α in oral squamous cell carcinoma was correlated with smoking, alcohol consumption, tumor size, differentiation degree and lymph node metastasis. The expression level of CCT-α protein was significantly increased in patients with a history of smoking and alcohol consumption （P=0.001, P=0.004）. With the increase of tumor diameter, the expression of CCT-α protein was significantly increased （P=0.005）. According to histopathological grade, the lower the degree of tumor differentiation, the higher the expression level of CCT-α protein （P=0.000）. The expression of CCT-α protein was significantly higher in patients with lymph node metastasis compared with no lymph node metastasis （P=0.000）. Quantitative real-time PCR results showed the CCT-α mRNA expression level was significantly higher in OSCC cells than that in normal oral epithelial cells （P=0.016）. The protein expression level of CCT-α was significantly higher in OSCC cells than that in normal oral epithelial cells. ConclusionsCCT-α may play a critical role in the carcinogenesis and development of OSCC.