黑木耳多糖模拟水解物的抗氧化及胃肠道分布规律研究

Studies on the Antioxidant Activity and Distribution in Gastrointestinal Tract of the Polysaccharide Hydrolysate from Auricularia auricula

探讨体外人工胃液肠水解黑木耳多糖片段的抗氧化活性及其在小鼠消化道内的动态分布规律。依次采用碱提醇沉,脱蛋白,人工胃肠液水解,透析,凝胶柱层析(Sephadex G100)得到5种黑木耳多糖组分(AAPF-1,AAPF-2,AAPF-3,AAPF-4,AAPF-5),利用体外OH·和DPPH·清除率和线虫H2O2抗性实验分析5种多糖的活性。利用异硫氰酸荧光素标记黑木耳多糖获得AAPF-1-Tyr-FITC,追踪其在小鼠胃肠道内24 h的动态分布规律。结果表明,5种黑木耳多糖组分中,AAPF-1(0.4 mg/m L)对OH·与DPPH·清除率均为最高,分别达68.54%与42.18%,并在60~150 min内均可显著延长线虫寿命。AAPF-1经胃很快排空到小肠,在小鼠小肠基本不吸收,而在大肠中的存留时间在24 h以上。

The purpose of this study was to investigate the antioxidant activity and the dynamic distribution of the digestive tract in mice of the Auricularia auricular polysaccharide fragments from the artificial gastrointestinal fluid in vitro. The 5 kinds of polysaccharides（AAPF-1,AAPF-2,AAPF-3,AAPF-4,AAPF-5） were separated from Auricularia auricular by alkali extraction and alcohol precipitation, deproteinization, artificial gastrointestinal fluid hydrolysis, dialysis and gel column chromatography（Sephadex G100）. OH· and DPPH· clearance in vitro and nematode H2 O2 resistance experiment of those 5 kinds of polysaccharides were analyzed. AAPF-1-Tyr-FITC was obtained by fluorescein labeled, and the dynamic distribution in the gastrointestinal tract of mice was studied. Results showed that AAPF-1（0.4 mg/m L） for OH· and DPPH· removal rate was the highest among 5 kinds of polysaccharides, that was 68.54% and 42.18% respectively, and the life span of the nematodes was significantly prolonged within 60~150 min. AAPF-1 was quickly evacuated to the small intestine, and not absorbed in the small intestine in mice, and the remaining time in the large intestine was more than 24 h.